通过联用效应导向分析和高分辨率质谱的多成像平面色谱法对40种肉桂的八种不同生物活性谱进行分析。

Eight different bioactivity profiles of 40 cinnamons by multi-imaging planar chromatography hyphenated with effect-directed assays and high-resolution mass spectrometry.

作者信息

Sumudu Chandana N G A S, Morlock Gertrud E

机构信息

Justus Liebig University Giessen, Institute of Nutritional Science, Chair of Food Science, and TransMIT Center for Effect-Directed Analysis, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.

出版信息

Food Chem. 2021 Feb 10;357:129135. doi: 10.1016/j.foodchem.2021.129135.

DOI:10.1016/j.foodchem.2021.129135

PMID:33905984

Abstract

Spices contain plenty of bioactive compounds, used to valorize foods. However, product quality may be affected by contaminations and adulterations along the global production chain. A newly developed multi-imaging in combination with bioactivity screening directly pointed to individual multi-potent compounds. For cinnamon as prominent example, the multi-imaging results provided a wealth of new information on their effects and clearly visualized the valorizing potential of cinnamon to foods. The separation focus was in the mid-polar to apolar range. Eight effect-directed assays (EDA, i.e. one radical scavenging, two biological and five biochemical assays) were performed in situ the high-performance thin-layer chromatography (HPTLC) adsorbent. Several multi-potent compound zones were revealed and further characterized by high-resolution mass spectrometry (HRMS), highlighting the bioactive potential of cinnamaldehyde, cinnamic acid, benzoic acid, coumarin, linoleic acid, oleic acid, stearic acid, palmitic acid, caproic acid, and linalool oxide. This HPTLC-UV/Vis/FLD-EDA-HRMS profiling provided comprehensive information on product quality and safety.

摘要

香料含有大量生物活性化合物，可用于提升食品价值。然而，产品质量可能会受到全球生产链中污染和掺假的影响。一种新开发的多成像技术与生物活性筛选相结合，直接指向了单个多效化合物。以肉桂为例，多成像结果提供了大量关于其效果的新信息，并清晰地展示了肉桂对食品的增值潜力。分离重点在中极性到非极性范围内。在高效薄层色谱（HPTLC）吸附剂上原位进行了八种效应导向分析（EDA，即一种自由基清除分析、两种生物学分析和五种生化分析）。揭示了几个多效化合物区域，并通过高分辨率质谱（HRMS）进一步表征，突出了肉桂醛、肉桂酸、苯甲酸、香豆素、亚油酸、油酸、硬脂酸、棕榈酸、己酸和氧化芳樟醇的生物活性潜力。这种HPTLC-UV/Vis/FLD-EDA-HRMS分析提供了有关产品质量和安全性的全面信息。