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RAP30/74: a general initiation factor that binds to RNA polymerase II.

作者信息

Burton Z F, Killeen M, Sopta M, Ortolan L G, Greenblatt J

机构信息

Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

出版信息

Mol Cell Biol. 1988 Apr;8(4):1602-13. doi: 10.1128/mcb.8.4.1602-1613.1988.

DOI:10.1128/mcb.8.4.1602-1613.1988
PMID:3380090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363320/
Abstract

We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for accurate transcription from the following promoters: the adenovirus major late promoter, the long terminal repeat of human immunodeficiency virus, P2 of the human c-myc gene, the mouse beta maj-globin promoter (all of which have TATA boxes), and the mouse dihydrofolate reductase promoter (which lacks a TATA box). RAP30/74 is not required for initiation by RNA polymerase III at the adenovirus virus-associated RNA promoters. Therefore, RAP30/74 is a general initiation factor that binds to RNA polymerase II.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/664b3b40abc9/molcellb00064-0239-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/2d8e2c6501ba/molcellb00064-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/b6847a4c876e/molcellb00064-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/f22b91855dfa/molcellb00064-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/29f3c1a05264/molcellb00064-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/664b3b40abc9/molcellb00064-0239-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/2d8e2c6501ba/molcellb00064-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/b6847a4c876e/molcellb00064-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/f22b91855dfa/molcellb00064-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/29f3c1a05264/molcellb00064-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a1/363320/664b3b40abc9/molcellb00064-0239-a.jpg

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RAP30/74: a general initiation factor that binds to RNA polymerase II.
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本文引用的文献

1
Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.纯化的RNA聚合酶II准确起始转录需要多种因素。
J Biol Chem. 1980 Dec 25;255(24):11992-6.
2
Mechanism of RNA polymerase II--specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts.RNA聚合酶II在体外特异性起始转录的机制：ATP需求与无帽延伸转录本
Cell. 1982 Jul;29(3):877-86. doi: 10.1016/0092-8674(82)90449-4.
3
Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
RNA 聚合酶 II 转录起始的结构基础。
Nat Rev Mol Cell Biol. 2015 Mar;16(3):129-43. doi: 10.1038/nrm3952. Epub 2015 Feb 18.
4
Functional interactions of the RNA polymerase II-interacting proteins Gdown1 and TFIIF.RNA 聚合酶 II 相互作用蛋白 Gdown1 和 TFIIF 的功能相互作用。
J Biol Chem. 2014 Apr 18;289(16):11143-11152. doi: 10.1074/jbc.M113.544395. Epub 2014 Mar 4.
5
RNA polymerase II transcription elongation control.RNA聚合酶II转录延伸控制
Chem Rev. 2013 Nov 13;113(11):8583-603. doi: 10.1021/cr400105n. Epub 2013 Aug 6.
6
Variants in the 3' UTR of general Transcription factor IIF, polypeptide 2 affect female calving efficiency in Japanese Black cattle.3'UTR 中的一般转录因子 IIF 多肽 2 变异与日本黑牛的母牛产犊效率有关。
BMC Genet. 2013 May 10;14:41. doi: 10.1186/1471-2156-14-41.
7
Rethinking the role of TFIIF in transcript initiation by RNA polymerase II.重新审视TFIIF在RNA聚合酶II转录起始中的作用。
Transcription. 2012 Jul-Aug;3(4):156-9. doi: 10.4161/trns.20725. Epub 2012 Jul 1.
8
The functions of TFIIF during initiation and transcript elongation are differentially affected by phosphorylation by casein kinase 2.TFIIF 在起始和转录延伸过程中的功能受到酪蛋白激酶 2 磷酸化的差异影响。
J Biol Chem. 2011 Jul 1;286(26):23160-7. doi: 10.1074/jbc.M110.205658. Epub 2011 May 12.
9
Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry.RNA 聚合酶 II-TFIIF 复合物的结构通过交联和质谱法揭示。
EMBO J. 2010 Feb 17;29(4):717-26. doi: 10.1038/emboj.2009.401. Epub 2010 Jan 21.
10
The Paf1 complex: platform or player in RNA polymerase II transcription?Paf1复合物：RNA聚合酶II转录中的平台还是参与者？
Biochim Biophys Acta. 2010 May-Jun;1799(5-6):379-88. doi: 10.1016/j.bbagrm.2010.01.001. Epub 2010 Jan 12.
介导RNA聚合酶II精确转录的因子的分离与特性分析。
J Biol Chem. 1982 Dec 10;257(23):14419-27.
4
Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.真核生物B类转录因子与启动子序列之间稳定前起始复合物的形成。
Nature. 1983 Feb 24;301(5902):680-6. doi: 10.1038/301680a0.
5
Cascades of Sigma factors.西格玛因子级联反应。
Cell. 1981 Sep;25(3):582-4. doi: 10.1016/0092-8674(81)90164-1.
6
Energy requirement for specific transcription initiation by the human RNA polymerase II system.人RNA聚合酶II系统特定转录起始的能量需求。
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7
Interactions between RNA polymerase II, factors, and template leading to accurate transcription.RNA聚合酶II、因子与模板之间的相互作用导致精确转录。
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8
A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.一种果蝇RNA聚合酶II转录因子含有启动子区域特异性DNA结合活性。
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9
The adenovirus major late promoter TATA box and initiation site are both necessary for transcription in vitro.腺病毒主要晚期启动子的TATA框和起始位点对于体外转录都是必需的。
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Cell. 1983 Oct;34(3):779-87. doi: 10.1016/0092-8674(83)90534-2.