Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Université de la Réunion, INSERM U1187, CNRS UMR 9192, IRD UMR 249, 94791 Sainte Clotilde, La Réunion, France.
Unité Mixte Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme Technologique CYROI, Université de la Réunion, INSERM U1188, 94791 Sainte Clotilde, La Réunion, France.
Int J Mol Sci. 2021 Mar 2;22(5):2495. doi: 10.3390/ijms22052495.
Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain-Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival.
由于缺乏疫苗和有效的抗病毒治疗，蚊媒传播的 Zika 病毒（ZIKV）已成为人类健康的真正威胁。该病毒最近引发了一场全球疫情，导致数百万人感染。ZIKV 并发症在患有格林-巴利综合征的成年人和患有越来越多先天性疾病的新生儿中突显出来，从轻度发育迟缓到致命情况不等。ZIKV 能够在包括肾脏在内的多种器官中建立长期感染的能力最近已经得到证实，但这种病毒感染的后果仍存在争议。我们的研究旨在确定 ZIKV 在肾细胞中的生长效率是否与葡萄糖浓度有关。用人肾 HK-2 细胞在正常和高葡萄糖浓度下感染不同的 ZIKV 株。病毒学检测表明，在高葡萄糖条件下，病毒复制减少，而不改变进入步骤（病毒结合、内化、融合）。与在正常葡萄糖浓度下感染 ZIKV 的 HK-2 细胞相比，这种复制减少与较低的病毒子代和增加的细胞活力相关。总之，我们首次表明，高葡萄糖水平会影响 ZIKV 的复制水平，并对肾脏细胞的存活产生影响。
