Shen Hailiang, Huang Linhua, Dou Huating, Yang Yali, Wu Houjiu
Citrus Research Institute, Southwest University, Chongqing 400000, China.
Citrus Research Institute, Chinese Academy of Agricultural Science, Chongqing 400000, China.
Nutrients. 2021 Mar 10;13(3):891. doi: 10.3390/nu13030891.
Trilobatin was identified as the primary bioactive component in the Rehd (LPR) leaves. This study explored the antiobesity effect of trilobatin from LPR leaves and its influence on gut microbiota in obese rats. Results showed that trilobatin could significantly reduce body and liver weight gain induced by a high-fat diet, and the accumulation of perirenal fat, epididymal fat, and brown fat of SD (Male Sprague-Dawley) obese rats in a dose-independent manner. Short-chain fatty acids (SCFAs) concentrations increased, especially the concentration of butyrate. Trilobatin supplementation could significantly increase the relative abundance of , , , , and , and decrease greatly the abundance of , , and , resulting in an increase of the ratio of to (except the genera of and ). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway predicted by the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) indicated the different relative metabolic pathways after trilobatin supplementation. This study may reveal the contribution of gut microbiota to the antiobesity effect of trilobatin from LPR leaves and predict the potential regulatory mechanism for obesity induced by a high-fat diet.
三叶苷被确定为火棘叶中的主要生物活性成分。本研究探讨了火棘叶中三叶苷的抗肥胖作用及其对肥胖大鼠肠道微生物群的影响。结果表明，三叶苷能以剂量非依赖性方式显著降低高脂饮食诱导的SD（雄性斯普拉格-道利）肥胖大鼠的体重和肝脏重量增加，以及肾周脂肪、附睾脂肪和棕色脂肪的积累。短链脂肪酸（SCFAs）浓度增加，尤其是丁酸盐浓度。补充三叶苷可显著增加[具体菌属未明确写出]、[具体菌属未明确写出]、[具体菌属未明确写出]、[具体菌属未明确写出]和[具体菌属未明确写出]的相对丰度，并显著降低[具体菌属未明确写出]、[具体菌属未明确写出]和[具体菌属未明确写出]的丰度，导致[具体菌属比值未明确写出]与[具体菌属比值未明确写出]的比值增加（除[具体菌属未明确写出]和[具体菌属未明确写出]属外）。通过未观察状态重建的群落系统发育研究（PICRUSt）预测的京都基因与基因组百科全书（KEGG）途径表明补充三叶苷后相对代谢途径不同。本研究可能揭示肠道微生物群对火棘叶中三叶苷抗肥胖作用的贡献，并预测高脂饮食诱导肥胖的潜在调节机制。
