Veziroglu Salih, Ayna Mustafa, Kohlhaas Theresa, Sayin Selin, Fiutowski Jacek, Mishra Yogendra Kumar, Karayürek Fatih, Naujokat Hendrik, Saygili Eyüp Ilker, Açil Yahya, Wiltfang Jörg, Faupel Franz, Aktas Oral Cenk, Gülses Aydin
Chair for Multicomponent Materials, Institute for Materials Science, Faculty of Engineering, Kiel University, Kaiserstr. 2, 24143 Kiel, Germany.
Department of Periodontology, University Hospital of Bonn, D-53111 Bonn, Germany.
Polymers (Basel). 2021 Mar 10;13(6):847. doi: 10.3390/polym13060847.
Biodegradable collagen-based materials have been preferred as scaffolds and grafts for diverse clinical applications in density and orthopedy. Besides the advantages of using such bio-originated materials, the use of collagen matrices increases the risk of infection transmission through the cells or the tissues of the graft/scaffold. In addition, such collagen-based solutions are not counted as economically feasible approaches due to their high production cost. In recent years, incorporation of marine algae in synthetic polymers has been considered as an alternative method for preparation grafts/scaffolds since they represent abundant and cheap source of potential biopolymers. Current work aims to propose a novel composite patch prepared by blending Sargassum vulgare powders (SVP) to polylactide (PLA) as an alternative to the porcine-derived membranes. SVP-PLA composite patches were produced by using a modified solvent casting method. Following detailed material characterization to assess the cytocompatibility, human osteoblasts (HOBs) and osteosarcoma cells (SaOS-2) were seeded on neat PLA and SVP-PLA patches. MTT and BrdU assays indicated a greater cytocompatibility and higher proliferation for HOBs cultured on SVP-PLA composite than for those cultured on neat PLA. SaOS-2 cells cultured on SVP-PLA exhibited a significant decrease in cell proliferation. The composite patch described herein exhibits an antiproliferative effect against SaOS-2 cells without impairing HOBs' adhesion and proliferation.
可生物降解的胶原基材料已成为密度和矫形外科各种临床应用中支架和移植物的首选。除了使用这种生物源材料的优点外，胶原基质的使用增加了通过移植物/支架的细胞或组织传播感染的风险。此外，由于其高生产成本，这种基于胶原的解决方案不被视为经济可行的方法。近年来，将海藻掺入合成聚合物中被认为是制备移植物/支架的一种替代方法，因为它们代表了丰富且廉价的潜在生物聚合物来源。当前的工作旨在提出一种通过将羊栖菜粉末（SVP）与聚乳酸（PLA）混合制备的新型复合贴片，作为猪源膜的替代品。SVP-PLA复合贴片采用改良的溶剂浇铸法制备。在进行详细的材料表征以评估细胞相容性后，将人成骨细胞（HOB）和骨肉瘤细胞（SaOS-2）接种在纯PLA和SVP-PLA贴片上。MTT和BrdU分析表明，在SVP-PLA复合材料上培养的HOB比在纯PLA上培养的具有更高的细胞相容性和增殖率。在SVP-PLA上培养的SaOS-2细胞的细胞增殖显著降低。本文所述的复合贴片对SaOS-2细胞具有抗增殖作用，而不损害HOB的粘附和增殖。
