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生乳奶酪提取物抗衰老作用的机制研究。

A Mechanistic Study of the Antiaging Effect of Raw-Milk Cheese Extracts.

机构信息

Université Clermont Auvergne, INRAE, VetAgro Sup, UMRF, F-15000 Aurillac, France.

Université Clermont Auvergne, CNRS, Clermont Auvergne INP, ICCF, F-63000 Clermont-Ferrand, France.

出版信息

Nutrients. 2021 Mar 10;13(3):897. doi: 10.3390/nu13030897.

DOI:10.3390/nu13030897
PMID:33802038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8000626/
Abstract

Many studies have highlighted the relationship between food and health status, with the aim of improving both disease prevention and life expectancy. Among the different food groups, fermented foods a have huge microbial biodiversity, making them an interesting source of metabolites that could exhibit health benefits. Our previous study highlighted the capacity of raw goat milk cheese, and some of the extracts recovered by the means of chemical fractionation, to increase the longevity of the nematode . In this article, we pursued the investigation with a view toward understanding the biological mechanisms involved in this phenomenon. Using mutant nematode strains, we evaluated the implication of the insulin-like DAF-2/DAF-16 and the p38 MAPK pathways in the phenomenon of increased longevity and oxidative-stress resistance mechanisms. Our results demonstrated that freeze-dried raw goat milk cheese, and its extracts, induced the activation of the DAF-2/DAF-16 pathway, increasing longevity. Concerning oxidative-stress resistance, all the extracts increased the survival of the worms, but no evidence of the implication of both of the pathways was highlighted, except for the cheese-lipid extract that did seem to require both pathways to improve the survival rate. Simultaneously, the cheese-lipid extract and the dried extract W70, obtained with water, were able to reduce the reactive oxygen species (ROS) production in human leukocytes. This result is in good correlation with the results obtained with the nematode.

摘要

许多研究都强调了食物与健康状况之间的关系，旨在改善疾病预防和预期寿命。在不同的食物组中，发酵食品具有巨大的微生物生物多样性，使它们成为具有健康益处的代谢物的有趣来源。我们之前的研究强调了生羊奶干酪的能力，以及通过化学分馏手段回收的一些提取物，可以延长线虫的寿命。在本文中，我们继续进行研究，以了解这一现象所涉及的生物学机制。使用突变型线虫品系，我们评估了胰岛素样 DAF-2/DAF-16 和 p38 MAPK 途径在长寿和抗氧化应激机制增强中的作用。我们的结果表明，冻干生羊奶干酪及其提取物诱导了 DAF-2/DAF-16 途径的激活，从而延长了寿命。关于抗氧化应激抗性，所有提取物都增加了蠕虫的存活率，但除了奶酪脂质提取物外，没有证据表明这两种途径都参与其中，而奶酪脂质提取物似乎需要这两种途径来提高存活率。同时，奶酪脂质提取物和用水获得的 W70 干燥提取物能够减少人白细胞中的活性氧（ROS）的产生。这一结果与线虫的结果非常吻合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/d1b6716ab861/nutrients-13-00897-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/438c59070031/nutrients-13-00897-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f32f5329eafe/nutrients-13-00897-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f20eb3bc0d19/nutrients-13-00897-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/28729c29d7ec/nutrients-13-00897-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/6f38d981a376/nutrients-13-00897-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/1153eda867ae/nutrients-13-00897-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f16582a60528/nutrients-13-00897-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/81d04b185b4a/nutrients-13-00897-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/7068788d5268/nutrients-13-00897-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/d1b6716ab861/nutrients-13-00897-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/438c59070031/nutrients-13-00897-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/5cce1d69a64d/nutrients-13-00897-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/cb0fc5732a9d/nutrients-13-00897-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f32f5329eafe/nutrients-13-00897-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f20eb3bc0d19/nutrients-13-00897-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/28729c29d7ec/nutrients-13-00897-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/6f38d981a376/nutrients-13-00897-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/1153eda867ae/nutrients-13-00897-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/f16582a60528/nutrients-13-00897-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/81d04b185b4a/nutrients-13-00897-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/7068788d5268/nutrients-13-00897-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ec2/8000626/d1b6716ab861/nutrients-13-00897-g012.jpg

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