Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, China.
Institute of Marine Drugs/School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, Nanning, China.
J Food Sci. 2024 Jun;89(6):3713-3728. doi: 10.1111/1750-3841.17069. Epub 2024 Apr 18.
Lactobacillus fermentum can exert antiaging effects, but their roles are strain-specific, and little is known about the molecular mechanisms in some strains. This study investigated the antiaging effects of L. fermentum WC2020 (WC2020) isolated from Chinese fermented pickles and the underlying mechanism of the action in Caenorhabditis elegans. WC2020 enhanced the mean lifespan of L1-stage and L4-stage worms by 22.67% and 12.42%, respectively, compared with Escherichia coli OP50 (OP50), a standard food source for C. elegans. WC2020-induced longevity was accompanied by an increase in body length and mitochondrial transmembrane potential and a reduction in lipid accumulation and the production of reactive oxygen species and malondialdehyde. Moreover, WC2020 increased the production of glutathione, superoxide dismutases, and catalases and altered the transcripts of many phenotype-related genes. Furthermore, WC2020-fed jnk-1 rather than akt-2 or pmk-1 loss-of-function mutants showed similar lifespans to OP50-fed worms. Correspondingly, WC2020 significantly upregulated the expression of jnk-1 rather than genes involved in insulin-like, p38 MAPK, bate-catenin, or TGF-beta pathway. Moreover, the increase in body length, mitochondrial transmembrane potential, and antioxidant capability and the decrease in lipid accumulation induced by WC2020 were not observed in jnk-1 mutants. Additionally, WC2020 increased the expression of daf-16 and the proportion of daf-16::GFP in the nucleus, and increased lifespan disappeared in WC2020-fed daf-16 loss-of-function mutants. In conclusion, WC2020 activated the JNK/DAF-16 pathway to improve mitochondria function, reduce oxidative stress, and then extend the longevity of nematodes, suggesting WC2020 could be a potential probiotic targeting JNK-mediated antioxidant pathway for antiaging in food supplements and bioprocessing. PRACTICAL APPLICATION: Aging has a profound impact on the global economy and human health and could be delayed by specific diets and nutrient resources. This study demonstrated that Lactobacillus fermentum WC2020 could be a potential probiotic strain used in food to promote longevity and health via the JNK-mediated antioxidant pathway.
发酵乳杆菌可发挥抗衰老作用，但作用因菌株而异，某些菌株的分子机制知之甚少。本研究探讨了从中国发酵泡菜中分离的发酵乳杆菌 WC2020（WC2020）的抗衰老作用及其在秀丽隐杆线虫中的作用机制。与秀丽隐杆线虫的标准食物源大肠杆菌 OP50（OP50）相比，WC2020 分别使 L1 期和 L4 期线虫的平均寿命延长了 22.67%和 12.42%。WC2020 诱导的长寿伴随着体长增加和线粒体跨膜电位增加，脂质积累减少，活性氧和丙二醛产生减少。此外，WC2020 增加了谷胱甘肽、超氧化物歧化酶和过氧化氢酶的产生，并改变了许多表型相关基因的转录。此外，jnk-1 而非 akt-2 或 pmk-1 功能丧失突变体喂养的 WC2020 表现出与 OP50 喂养的线虫相似的寿命。相应地，WC2020 显著上调了 jnk-1 而非参与胰岛素样、p38 MAPK、bate-catenin 或 TGF-beta 途径的基因的表达。此外，在 jnk-1 突变体中未观察到 WC2020 诱导的体长增加、线粒体跨膜电位增加和抗氧化能力增加以及脂质积累减少。此外，WC2020 增加了 daf-16 的表达和核内 daf-16::GFP 的比例，并且在 WC2020 喂养的 daf-16 功能丧失突变体中，延长寿命的现象消失。总之，WC2020 通过激活 JNK/DAF-16 途径来改善线粒体功能，减少氧化应激，从而延长线虫的寿命，这表明 WC2020 可以作为一种潜在的益生菌，通过 JNK 介导的抗氧化途径靶向食品补充剂和生物加工中的抗衰老。实际应用：衰老对全球经济和人类健康有深远的影响，可以通过特定的饮食和营养资源来延缓。本研究表明，发酵乳杆菌 WC2020 可作为一种潜在的益生菌菌株，通过 JNK 介导的抗氧化途径在食品中促进长寿和健康。
