Jung Young Yun, Ko Jeong-Hyeon, Um Jae-Young, Sethi Gautam, Ahn Kwang Seok
Department of Science in Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117600, Singapore.
Cancers (Basel). 2021 Mar 17;13(6):1347. doi: 10.3390/cancers13061347.
The potential effects of bergamotiin (BGM) on the suppression of cancer cachexia was evaluated under in vitro and in vivo conditions to investigate its possible inhibitory effects on the muscle and fat loss.
The differentiated C2C12 and 3T3L1 cells were treated with BGM after the induction of cancer-cachexia with pancreatic cancer conditioned media (CM). The expression levels of the various molecules involved in the differentiation and loss of muscle and fat (MuRF-1, Atrogin-1, C/EBPα, and PPARγ) were analyzed by Western blot and oil red O staining. For in vivo experiment, MIA PaCa-2 cells were injected into the mice ( = 6), and then BGM (1 mg/kg) was intraperitoneally administered to analyze muscle and adipose tissue by Hematoxylin and Eosin staining and Western blot.
BGM displayed a significant effect on the inhibition of muscle and fat catabolism under both in vitro and in vivo conditions. The results of the in vivo experiment revealed a remarkable suppressive effect of BGM on the weight loss in mice.
The potential effects of BGM on the inhibition of muscle and fat catabolism in vitro and in vivo were thus confirmed. Based on the results, the impact of BGM on cancer cachexia could be possibly analyzed in the future clinical studies.
在体外和体内条件下评估佛手柑素（BGM）对抑制癌症恶病质的潜在作用，以研究其对肌肉和脂肪流失的可能抑制作用。
在用胰腺癌条件培养基（CM）诱导癌症恶病质后，用BGM处理分化的C2C12和3T3L1细胞。通过蛋白质免疫印迹法和油红O染色分析参与肌肉和脂肪分化及流失的各种分子（MuRF-1、Atrogin-1、C/EBPα和PPARγ）的表达水平。对于体内实验，将MIA PaCa-2细胞注射到小鼠体内（n = 6），然后腹腔注射BGM（1 mg/kg），通过苏木精和伊红染色及蛋白质免疫印迹法分析肌肉和脂肪组织。
在体外和体内条件下，BGM均对抑制肌肉和脂肪分解代谢具有显著作用。体内实验结果显示，BGM对小鼠体重减轻具有显著的抑制作用。
因此证实了BGM在体外和体内对抑制肌肉和脂肪分解代谢的潜在作用。基于这些结果，未来的临床研究中可能会分析BGM对癌症恶病质的影响。
