Rodríguez-Gómez Citlali, Durán-Riveroll Lorena María, Okolodkov Yuri B, Oliart-Ros Rosa María, García-Casillas Andrea M, Cembella Allan D
Unidad de Investigación y Desarrollo en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Veracruz, Veracruz 91897, Mexico.
CONACYT-Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada, Carretera Tijuana-Ensenada 3918, Ensenada 22860, Baja California, Mexico.
Microorganisms. 2021 Mar 17;9(3):619. doi: 10.3390/microorganisms9030619.
Bacterial diversity was explored among field samples and cultured isolates from coral reefs within the Veracruz Reef System. Bacterioplankton and bacteriobenthos were characterized by pyrosequencing 16S rRNA genes. Identified sequences belonged to the kingdom Bacteria and classified into 33 phyla. Proteobacteria (likely SAR11 clade) dominated in collective field samples, whereas Firmicutes were the most abundant taxa among cultured isolates. Bioinformatic sorting of sequences to family level revealed 223 bacterial families. Pseudomonadaceae, Exiguobacteraceae and Bacillaceae were dominant among cultured isolates. Vibrionaceae, Alteromonadaceae, and Flavobacteriaceae dominated in reef-associated sediments, whereas Rickettsiaceae and Synechoccaceae were more highly represented in the water column. Bacterial communities from sediments were more diverse than from the water column. This study reveals cryptic bacterial diversity among microenvironmental components of marine microbial reef communities subject to differential influence of anthropogenic stressors. Such investigations are critical for constructing scenarios of environmentally induced shifts in bacterial biodiversity and species composition.
在韦拉克鲁斯珊瑚礁系统内的珊瑚礁野外样本和培养分离物中探索了细菌多样性。通过对16S rRNA基因进行焦磷酸测序来表征浮游细菌和底栖细菌。鉴定出的序列属于细菌界，分为33个门。变形菌门（可能是SAR11进化枝）在野外样本中占主导地位，而厚壁菌门是培养分离物中最丰富的分类群。将序列进行生物信息学分类到科水平，共揭示了223个细菌科。假单胞菌科、微小杆菌科和芽孢杆菌科在培养分离物中占主导地位。弧菌科、交替单胞菌科和黄杆菌科在与珊瑚礁相关的沉积物中占主导地位，而立克次氏体科和聚球藻科在水柱中占比更高。沉积物中的细菌群落比水柱中的细菌群落更加多样。这项研究揭示了海洋微生物珊瑚礁群落微环境组分中隐秘的细菌多样性，这些微环境组分受到人为压力源的不同影响。此类调查对于构建环境诱导的细菌生物多样性和物种组成变化的情景至关重要。
