Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
cGMP Regenerative Medicine Facility, Department of Physiology and Pharmacology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Int J Mol Sci. 2021 Mar 17;22(6):3086. doi: 10.3390/ijms22063086.
Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering. Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties. Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized. The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells. The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.
合成聚合物和天然聚合物的混合复合材料是骨组织工程的首选材料。由于最近有报道称其具有成骨性，因此，具有生物活性的海洋硫酸多糖岩藻聚糖在新型生物医学支架的开发中引起了极大的兴趣。本文制备并表征了一系列含有岩藻聚糖的聚己内酯杂化支架，其可以单独存在，也可以与 κ-卡拉胶和硫酸软骨素混合存在。确定了制备方法和多糖组成对其形态以及机械、热、吸水率和孔隙率性能的影响，同时通过评估接种人脂肪间充质干细胞的细胞黏附、活力和成骨分化来研究其成骨诱导潜力。结果证实了岩藻聚糖的成骨能力，表明其掺入聚己内酯基质中有效地促进了细胞附着和活力，从而证实了其在用于骨组织再生应用的生物医学支架开发中的潜力。
