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口腔角质形成细胞和THP-1巨噬细胞中细胞因子表达对最常见血清型反应的比较分析 。（原文结尾处不完整，推测可能是想表达某种细菌或病毒的最常见血清型，这里按完整的句子结构进行了翻译）

Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of .

作者信息

Betancur Daniel, Muñoz Grez Camila, Oñate Angel

机构信息

Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, Chile.

出版信息

Microorganisms. 2021 Mar 17;9(3):622. doi: 10.3390/microorganisms9030622.

DOI:10.3390/microorganisms9030622
PMID:33802988
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8002688/
Abstract

BACKGROUND

Periodontitis is a chronic inflammatory disease associated with a dysbiotic biofilm. Many pathogens have been related with its progression and severity, one of which is , a Gram-negative bacteria with seven serotypes (a-g) according with the structure of its LPS, with serotype b defined as the most virulent compared with the other serotypes. The aim of this study was to evaluate the response of oral keratinocytes and macrophages to .

METHODS

Oral keratinocytes (OKF6/TERT2) and macrophages (THP-1) were infected with serotypes a, b and c. The expression of IL-1β, IL-6, IL-8, IL-18, TNF-α, MMP-9, RANKL, TLR-2, TLR-4, TLR-6, thymic stromal lymphopoietin (TSLP), and ICAM-1 was evaluated by qPCR at 2 and 24 h after infection.

RESULTS

An increase in the expression of these molecules was induced by all serotypes at both times of infection, with macrophages showing higher levels of expression at 24 h compared to epithelial cells in which the highest levels were observed in the first hours after infection.

CONCLUSIONS

Keratinocytes and macrophages contribute to the inflammation in periodontitis from the early stages of infection, producing the first waves of cytokines, acting as the first signal for professional immune cell recruitment and modulation of more specific immune responses.

摘要

背景

牙周炎是一种与生态失调生物膜相关的慢性炎症性疾病。许多病原体与其进展和严重程度有关，其中之一是 ，这是一种革兰氏阴性菌，根据其脂多糖结构有七种血清型（a - g），与其他血清型相比，血清型b被定义为毒性最强的。本研究的目的是评估口腔角质形成细胞和巨噬细胞对 的反应。

方法

用 血清型a、b和c感染口腔角质形成细胞（OKF6/TERT2）和巨噬细胞（THP - 1）。在感染后2小时和24小时通过qPCR评估白细胞介素 - 1β（IL - 1β）、白细胞介素 - 6（IL - 6）、白细胞介素 - 8（IL - 8）、白细胞介素 - 18（IL - 18）、肿瘤坏死因子 - α（TNF - α）、基质金属蛋白酶 - 9（MMP - 9）、核因子κB受体活化因子配体（RANKL）、Toll样受体2（TLR - 2）、Toll样受体4（TLR - 4）、Toll样受体6（TLR - 6）、胸腺基质淋巴细胞生成素（TSLP）和细胞间黏附分子 - 1（ICAM - 1）的表达。

结果

在两个感染时间点，所有血清型均诱导这些分子表达增加，与上皮细胞相比，巨噬细胞在24小时时表达水平更高，上皮细胞在感染后的最初几小时观察到最高水平。

结论

角质形成细胞和巨噬细胞在感染早期就参与牙周炎的炎症反应，产生首批细胞因子浪潮，作为专业免疫细胞募集和更特异性免疫反应调节的首个信号。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/575a0b022b82/microorganisms-09-00622-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/439bf6730ba5/microorganisms-09-00622-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/7359f1bcfe7d/microorganisms-09-00622-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/28a0c123740a/microorganisms-09-00622-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/145cd825a0d8/microorganisms-09-00622-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/bdd42b24178f/microorganisms-09-00622-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/c9aca7be4d60/microorganisms-09-00622-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/2eac9c5b1e05/microorganisms-09-00622-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/575a0b022b82/microorganisms-09-00622-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/439bf6730ba5/microorganisms-09-00622-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/7359f1bcfe7d/microorganisms-09-00622-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/28a0c123740a/microorganisms-09-00622-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/145cd825a0d8/microorganisms-09-00622-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/bdd42b24178f/microorganisms-09-00622-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/c9aca7be4d60/microorganisms-09-00622-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/2eac9c5b1e05/microorganisms-09-00622-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa52/8002688/575a0b022b82/microorganisms-09-00622-g008.jpg

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