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针对最常见血清型的巨噬细胞极化标志物的表达

Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes of .

作者信息

Betancur Daniel, Muñoz Camila, Oñate Angel

机构信息

Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción 4030000, Chile.

Discipline of Periodontology, Department of Surgical Stomatology, Faculty of Dentistry, Universidad de Concepción, Concepción 4030000, Chile.

出版信息

Microorganisms. 2022 Jul 9;10(7):1384. doi: 10.3390/microorganisms10071384.

DOI:10.3390/microorganisms10071384
PMID:35889103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9318388/
Abstract

UNLABELLED

, a Gram-negative bacterium with seven serotypes (a-g) according to the structure of its LPS, has been defined as one of the most important pathogens in the development of a dysbiotic periodontal biofilm and the onset of periodontitis (an inflammatory chronic disease of the tissues around the teeth), where the serotype b is characterized as the most virulent compared with the other serotypes. The aim of this study was to evaluate the expression of the macrophage polarization markers M0, M1, and M2 against .

METHODS

THP-1 cells were infected with serotypes a, b, and c. The expression of CD11b, CD4, CD14, and CD68 for M0; IL-6, HLA/DRA, and CXCL10 for M21, and IL-10, CD163, fibronectin-1 or FN1, and CCL17 was evaluated by qPCR at 2 and 24 h after infection.

RESULTS

An increase in the expression of these molecules was induced by all serotypes at both times of infection, showing higher levels of expression to the M1 panel at 2 and 24 h compared to other markers.

CONCLUSIONS

has a role in the macrophage polarization to the M1 phenotype in a non-serotype-dependent manner.

摘要

未标记

，一种革兰氏阴性菌，根据其脂多糖结构有七种血清型（a - g），已被定义为在生态失调的牙周生物膜形成和牙周炎（牙齿周围组织的一种慢性炎症性疾病）发病过程中最重要的病原体之一，其中血清型b被认为是与其他血清型相比毒性最强的。本研究的目的是评估巨噬细胞极化标志物M0、M1和M2对 的表达情况。

方法

用血清型a、b和c感染THP - 1细胞。在感染后2小时和24小时通过qPCR评估M0的CD11b、CD4、CD14和CD68；M1的IL - 6、HLA/DRA和CXCL10，以及IL - 10、CD163、纤连蛋白 - 1或FN1和CCL17的表达。

结果

在两个感染时间点，所有血清型均诱导这些分子的表达增加，与其他标志物相比，在2小时和24小时时M1组的表达水平更高。

结论

以非血清型依赖的方式在巨噬细胞向M1表型的极化中起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/01b3e763f23a/microorganisms-10-01384-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/39ba50607131/microorganisms-10-01384-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/dbddda710ee4/microorganisms-10-01384-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/9595608e034d/microorganisms-10-01384-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/01b3e763f23a/microorganisms-10-01384-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/39ba50607131/microorganisms-10-01384-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/dbddda710ee4/microorganisms-10-01384-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/9595608e034d/microorganisms-10-01384-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de59/9318388/01b3e763f23a/microorganisms-10-01384-g004.jpg

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本文引用的文献

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Medicina (Kaunas). 2022 Apr 13;58(4):537. doi: 10.3390/medicina58040537.
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Outer Membrane Vesicles From Switch M0-Like Macrophages Toward the M1 Phenotype to Destroy Periodontal Tissues in Mice.来自类似M0开关巨噬细胞的外膜囊泡促使其向M1表型转变，从而破坏小鼠的牙周组织。
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Granulocyte-macrophage colony-stimulating factor (GM-CSF) in subjects with different stages of periodontitis according to the new classification.
根据新分类，不同牙周炎阶段患者的粒-巨噬细胞集落刺激因子（GM-CSF）。
J Appl Oral Sci. 2022 Mar 9;30:e20210423. doi: 10.1590/1678-7757-2021-0423. eCollection 2022.
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LPS binds human interleukin-8.脂多糖结合人白细胞介素-8。
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Pro-inflammatory polarization primes Macrophages to transition into a distinct M2-like phenotype in response to IL-4.促炎极化使巨噬细胞在 IL-4 的作用下向独特的 M2 样表型转化。
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Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes of .口腔角质形成细胞和THP-1巨噬细胞中细胞因子表达对最常见血清型反应的比较分析 。（原文结尾处不完整，推测可能是想表达某种细菌或病毒的最常见血清型，这里按完整的句子结构进行了翻译）
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