van Zwieten E J, Ravid R, Swaab D F, Van de Woude T
Netherlands Institute for Brain Research, Amsterdam.
Brain Res. 1988 Dec 6;474(2):369-73. doi: 10.1016/0006-8993(88)90453-2.
Following two weeks of cerebroventricular administration of arginine-vasopressin (AVP) by Accurel implants, two types of binding sites for this peptide were immunocytochemically visualized in blood vessels in the brain of Brattleboro (di/di) rats: (1) endothelial cells of capillaries were stained with the highest density in hippocampus, striatum, and locus coeruleus (LC), whereas only few such stained cells were present in the septum and cerebral cortex. (2) Bound AVP was also present on endothelial cells and pericyte-like cells in larger blood vessels in striatum and the LC. Both types of vasopressin binding site staining on blood vessels were dose-dependent and could be further enhanced by additional in vitro preincubation of the fixed sections with AVP. This staining was not present in rats implanted with either oxytocin or alpha-melanocyte-stimulating hormone.
通过Accurel植入物对Brattleboro（di/di）大鼠进行为期两周的脑室注射精氨酸加压素（AVP）后，在其大脑血管中通过免疫细胞化学方法观察到该肽的两种结合位点：（1）毛细血管内皮细胞在海马、纹状体和蓝斑（LC）中染色密度最高，而在隔区和大脑皮层中只有少数此类染色细胞。（2）结合的AVP也存在于纹状体和LC中较大血管的内皮细胞和类周细胞上。血管上两种类型的加压素结合位点染色均呈剂量依赖性，并且通过用AVP对固定切片进行额外的体外预孵育可进一步增强。在植入催产素或α-黑素细胞刺激素的大鼠中未出现这种染色。
