Costa Thais Correia, Du Min, Nascimento Karolina Batista, Galvão Matheus Castilho, Meneses Javier Andrés Moreno, Schultz Erica Beatriz, Gionbelli Mateus Pies, Duarte Marcio de Souza
Department of Animal Science, Universidade Federal de Viçosa, Viçosa MG 3657-000, Brazil.
Muscle Biology and Nutrigenomics Laboratory, Universidade Federal de Viçosa, Viçosa MG 3657-000, Brazil.
Animals (Basel). 2021 Mar 18;11(3):860. doi: 10.3390/ani11030860.
We aimed to investigate the effects of maternal protein restriction during mid-gestation on the skeletal muscle composition of the offspring. In the restriction treatment (RES, n = 9), cows were fed a basal diet, while in the control (CON, n = 9) group cows received the same RES diet plus the protein supplement during mid-gestation (100-200d). Samples of muscle were collected from the offspring at 30d and 450d postnatal. Muscle fiber number was found to be decreased as a result of maternal protein restriction and persisted throughout the offspring's life ( < 0.01). The collagen content was enhanced ( < 0.05) due to maternal protein restriction at 30d. mRNA expression tended to be higher ( = 0.08) in RES 30d offspring, however, no difference ( > 0.05) was found among treatments at 450d. Taken together, our results suggest that maternal protein restriction during mid-gestation has major and persistent effects by reducing muscle fiber formation and may slightly increase collagen accumulation in the skeletal muscle of the offspring. Although maternal protein restriction may alter the muscle fiber metabolism by favoring the establishment of a predominant glycolytic metabolism, the postnatal environment may be a determinant factor that establishes the different proportion of muscle fiber types.
我们旨在研究妊娠中期母体蛋白质限制对后代骨骼肌组成的影响。在限制处理组（RES，n = 9）中，母牛饲喂基础日粮，而在对照组（CON，n = 9）中，母牛在妊娠中期（100 - 200天）接受相同的RES日粮并添加蛋白质补充剂。在出生后30天和450天从后代采集肌肉样本。发现由于母体蛋白质限制，肌纤维数量减少，并且在后代的整个生命过程中持续存在（P < 0.01）。由于妊娠中期母体蛋白质限制，在30天时胶原蛋白含量增加（P < 0.05）。RES 30天后代的mRNA表达倾向于更高（P = 0.08），然而，在450天时各处理之间未发现差异（P > 0.05）。综上所述，我们的结果表明，妊娠中期母体蛋白质限制通过减少肌纤维形成具有重大且持久的影响，并且可能会略微增加后代骨骼肌中的胶原蛋白积累。尽管母体蛋白质限制可能通过促进主要糖酵解代谢的建立来改变肌纤维代谢，但出生后的环境可能是决定肌纤维类型不同比例的一个决定性因素。
