Song Da Hye, Chun Byung Hee, Lee Sunmin, Son Su Young, Reddy Chagam Koteswara, Mun Ha In, Jeon Che Ok, Lee Choong Hwan
Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.
Department of Life Science, Chung-Ang University, Seoul 06974, Korea.
Foods. 2021 Mar 18;10(3):641. doi: 10.3390/foods10030641.
and are secondary fermented soybean products from (primary fermented product) following a complex fermentation process that separates the products into solid () and liquid () states. We performed a comparative study on gas chromatography mass spectrometry-(GC-MS) and liquid chromatography mass spectrometry-(LC-MS) based metabolite profiling with fungal and bacterial microbial community analysis of and during fermentation. Metabolite profiling and microbial community data showed distinct patterns, depending on the fermentation process. The relative levels of metabolic patterns were similar and most of the microorganisms produced halophilic or halotolerant microbes during the fermentation period in and . In the end products, isoflavones, soyasaponins, and amino acids were largely distributed and and were dominant, whereas the biogenic amine and phenylpropanoid contents were highly distributed in the end products, with higher levels of and . Our results demonstrate that the quality of and is predominantly influenced by the microbiome and by metabolite changes during fermentation. Moreover, the present study provides a platform for comparing samples in different states.
纳豆和味噌是由纳豆(初级发酵产品)经过复杂发酵过程制成的二级发酵大豆产品,该过程将产品分离为固态(纳豆)和液态(味噌)状态。我们对纳豆和味噌发酵过程中基于气相色谱 - 质谱联用(GC - MS)和液相色谱 - 质谱联用(LC - MS)的代谢物谱分析以及真菌和细菌微生物群落分析进行了比较研究。代谢物谱分析和微生物群落数据显示出不同的模式,这取决于发酵过程。代谢模式的相对水平相似,并且在纳豆和味噌的发酵期,大多数微生物产生嗜盐或耐盐微生物。在纳豆最终产品中,异黄酮、大豆皂苷和氨基酸大量分布,纳豆激酶和纳豆菌占主导,而生物胺和苯丙烷类化合物含量在味噌最终产品中高度分布,且酪胺和香豆酸水平较高。我们的结果表明,纳豆和味噌的质量主要受微生物群落和发酵过程中代谢物变化的影响。此外,本研究提供了一个比较不同状态样品的平台。