Catambacan Dennice G, Cumagun Christian Joseph R
Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture and Food Science, University of the Philippines, Los Baños 4031, Laguna, Philippines.
Research Information and Compliance Division, TADECO, Panabo City 8105, Davao del Norte, Philippines.
J Fungi (Basel). 2021 Mar 18;7(3):224. doi: 10.3390/jof7030224.
The antagonistic activity of fungal endophytes isolated from weeds growing in Cavendish banana farms was determined against f. sp. TR4 ( TR4) causing Fusarium wilt of Cavendish banana. Forty-nine out of the total 357 fungal endophytes from the roots of weeds exhibited antagonistic activity against TR4. High inhibitory activity at 79.61-99.31% based on dual culture assay was recorded in endophytes TDC029, TDC075, sp. TDC037, sp. TDC241, and sp. TDC474. All five endophytes were identified through DNA sequencing with 86-100% identity. Endophyte-treated Grand Naine and GCTCV 218 plantlets showed significantly lower disease incidence ( = 0.014), significantly lower degree of leaf yellowing ( = 0.037) and rhizome discoloration ( = 0.003). In addition, the cultivar Grand Naine was consistently highly susceptible compared with the tolerant cultivar GCTCV 218.
测定了从卡文迪什香蕉种植园杂草中分离出的真菌内生菌对引起卡文迪什香蕉枯萎病的尖孢镰刀菌古巴专化型（TR4）的拮抗活性。从杂草根部的357株真菌内生菌中，有49株对TR4表现出拮抗活性。基于对峙培养法，内生菌TDC029、TDC075、某菌TDC037、某菌TDC241和某菌TDC474的抑制活性高达79.61%-99.31%。通过DNA测序鉴定了所有五株内生菌，其序列一致性为86%-100%。经内生菌处理的大麦克和GCTCV 218组培苗的发病率显著降低（P = 0.014），叶片黄化程度（P = 0.037）和根茎变色程度（P = 0.003）也显著降低。此外，与耐性品种GCTCV 218相比，大麦克品种始终高度感病。
