Yang Yun Xuan, Wu Vicky, Malak Hadi, Ahamed Aliya Peer, Lo Aaron, Abraham Yannis, Miller Catherine
College of Medicine and Dentistry, James Cook University, Cairns, QLD 4870, Australia.
College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia.
Dent J (Basel). 2021 Mar 1;9(3):26. doi: 10.3390/dj9030026.
The aim of this study was to evaluate the effect of varying concentrations of a turmeric solution on the growth rates of oral bacteria sampled from dental students.
Bacterial cultures were grown overnight in aerobic conditions from plaque samples obtained from five test subjects. With the exception of the control, samples were exposed to different treatments; including chlorhexidine gluconate 2 mg/mL, prepared turmeric solution (TS) mouthwash: TS 0.25 mL (7.375 mg/mL), TS 0.5 mL (14.75 mg/mL), and TS 1 mL (29.50 mg/mL). Growth rate of the bacterial cultures were assessed by monitoring changes in optical density readings at 600 nm at hourly intervals for a six-hour period. The data were plotted and the exponential trend was used to calculate individual rates of growth. Data was analyzed using a one-way ANOVA with the significance confirmed using the Tukey-HSD test.
Growth observed in the bacteria exposed to the turmeric solution, was significantly greater ( < 0.05) when compared with the bacteria exposed to the medium alone. There was a significant difference found between the bacterial growth rate of the 1 mL turmeric solution against the growth rate of the bacteria in the 0.25 and 0.5 mL turmeric solutions.
Comparison of growth rates of oral bacteria suggested that turmeric solutions of concentrations between 7.357 and 29.5 mg/mL (0.25-1 mL) were unlikely to exhibit bacteriostatic or bactericidal properties, and, conversely, increased bacterial growth. Considering this result, it is unlikely that turmeric mouthwash made from store-bought turmeric would have any antibacterial effects against oral bacteria, and may even promote bacterial growth.
本研究旨在评估不同浓度姜黄溶液对从牙科学生采集的口腔细菌生长速率的影响。
从五名受试对象获取的牙菌斑样本在有氧条件下进行过夜细菌培养。除对照组外，样本接受不同处理；包括2 mg/mL葡萄糖酸洗必泰、配制的姜黄溶液（TS）漱口水：TS 0.25 mL（7.375 mg/mL）、TS 0.5 mL（14.75 mg/mL）和TS 1 mL（29.50 mg/mL）。通过在六小时内每隔一小时监测600 nm处光密度读数的变化来评估细菌培养物的生长速率。绘制数据并使用指数趋势计算个体生长速率。使用单因素方差分析进行数据分析，并使用Tukey-HSD检验确认显著性。
与仅暴露于培养基的细菌相比，暴露于姜黄溶液的细菌中观察到的生长显著更高（<0.05）。发现1 mL姜黄溶液的细菌生长速率与0.25 mL和0.5 mL姜黄溶液中细菌的生长速率之间存在显著差异。
口腔细菌生长速率的比较表明，浓度在7.357至29.5 mg/mL（0.25 - 1 mL）之间的姜黄溶液不太可能表现出抑菌或杀菌特性，相反，会增加细菌生长。考虑到这一结果，用市售姜黄制成的姜黄漱口水不太可能对口腔细菌有任何抗菌作用，甚至可能促进细菌生长。
