College of Forestry, Northwest A&F University, Yangling 712100, China.
College of Life Sciences, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2021 Mar 25;22(7):3353. doi: 10.3390/ijms22073353.
Bunge has been widely used in the treatment of cardiovascular and cerebrovascular diseases, due to the pharmacological action of its active components such as the tanshinones. Plasma membrane (PM) H-ATPase plays key roles in numerous physiological processes in plants. However, little is known about the PM H-ATPase gene family in (). Here, nine PM H-ATPase isoforms were identified and named . Phylogenetic tree analysis showed that the genetic distance of was relatively far in the PM H-ATPase family. Moreover, the transmembrane structures were rich in protein. In addition, was found to be highly expressed in roots and flowers. HPLC revealed that accumulation of dihydrotanshinone (DT), cryptotanshinone (CT), and tanshinone I (TI) was significantly reduced in the lines but was increased in the lines, ranging from 2.54 to 3.52, 3.77 to 6.33, and 0.35 to 0.74 mg/g, respectively, suggesting that is a candidate regulator of tanshinone metabolites. Moreover, qRT-PCR confirmed that the expression of tanshinone biosynthetic-related key enzymes was also upregulated in the lines. In summary, this study highlighted PM H-ATPase function and provided new insights into regulatory candidate genes for modulating secondary metabolism biosynthesis in .
Bunge 已被广泛应用于治疗心血管和脑血管疾病，这归因于其活性成分如丹参酮的药理学作用。质膜（PM）H+-ATPase 在植物的许多生理过程中发挥着关键作用。然而，人们对（）中的 PM H+-ATPase 基因家族知之甚少。在这里，鉴定并命名了 9 种 PM H+-ATPase 同工型。系统发育树分析表明，在 PM H+-ATPase 家族中，的遗传距离相对较远。此外，蛋白富含跨膜结构。另外，发现其在根和花中高度表达。HPLC 表明，在 株系中，二氢丹参酮（DT）、隐丹参酮（CT）和丹参酮 I（TI）的积累明显减少，而在 株系中增加，范围分别为 2.54 至 3.52、3.77 至 6.33 和 0.35 至 0.74 mg/g，表明 是丹参酮代谢物的候选调节剂。此外，qRT-PCR 证实，丹参酮生物合成相关关键酶的表达也在 株系中上调。综上所述，本研究强调了 PM H+-ATPase 的功能，并为调节次生代谢物生物合成的候选调控基因提供了新的见解。
