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丹参酮和丹酚酸的生物合成在毛状根中受[此处原文缺失相关调节因子]调节。

Tanshinone and salvianolic acid biosynthesis are regulated by in hairy roots.

作者信息

Hao Xiaolong, Pu Zhongqiang, Cao Gang, You Dawei, Zhou Yang, Deng Changping, Shi Min, Nile Shivraj Hariram, Wang Yao, Zhou Wei, Kai Guoyin

机构信息

Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China.

Institute of Plant Biotechnology, School of Life Sciences, Shanghai Normal University, Shanghai 200234, PR China.

出版信息

J Adv Res. 2020 Jan 25;23:1-12. doi: 10.1016/j.jare.2020.01.012. eCollection 2020 May.

DOI:10.1016/j.jare.2020.01.012
PMID:32071787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7016019/
Abstract

Bunge is an herb rich in bioactive tanshinone and salvianolic acid compounds. It is primarily used as an effective medicine for treating cardiovascular and cerebrovascular diseases. Liposoluble tanshinones and water-soluble phenolic acids are a series of terpenoids and phenolic compounds, respectively. However, the regulation mechanism for the simultaneous promotion of tanshinone and salvianolic acid biosynthesis remains unclear. This study identified a R2R3-MYB subgroup 20 transcription factor (TF), , which was predominantly expressed in lateral roots. The accumulation of major bioactive metabolites, tanshinones, and salvianolic acids, was improved in overexpression (OE) hairy root lines, but reduced in knockout (KO) lines. The qRT-PCR analysis revealed that the transcriptional expression levels of tanshinone and salvianolic acid biosynthesis genes were upregulated by and downregulated by . Dual-Luciferase (Dual-LUC) assays demonstrated that significantly activated the transcription of , , and . These results suggest that can promote tanshinone and salvianolic acid production. The present findings illustrate the exploitation of R2R3-MYB in terpenoid and phenolic biosynthesis, as well as provide a feasible strategy for improving tanshinone and salvianolic acid contents by MYB proteins in .

摘要

丹参是一种富含生物活性丹参酮和丹酚酸类化合物的草本植物。它主要用作治疗心脑血管疾病的有效药物。脂溶性丹参酮和水溶性酚酸分别是一系列萜类化合物和酚类化合物。然而，同时促进丹参酮和丹酚酸生物合成的调控机制仍不清楚。本研究鉴定出一个R2R3-MYB亚组20转录因子（TF），其主要在侧根中表达。在过表达（OE）毛状根系中，主要生物活性代谢产物丹参酮和丹酚酸的积累有所改善，但在敲除（KO）系中则减少。qRT-PCR分析表明，丹参酮和丹酚酸生物合成基因的转录表达水平在[具体基因名称]过表达时上调，在敲除时下调。双荧光素酶（Dual-LUC）分析表明，[具体基因名称]显著激活了[相关基因名称1]、[相关基因名称2]和[相关基因名称3]的转录。这些结果表明，[具体基因名称]可以促进丹参酮和丹酚酸的产生。本研究结果阐明了R2R3-MYB在萜类和酚类生物合成中的应用，也为通过[植物名称]中的MYB蛋白提高丹参酮和丹酚酸含量提供了一种可行的策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/5146b169ad6c/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/b4c8d360c7d4/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/02292d9fcbde/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/9e213b99c3d8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/1a3b5855e7fb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/faf65d286ef6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/2703318c0308/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/fca2c9f58078/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/f6f75b38eaab/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/fc785f8b5088/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/f5d59f1f6413/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/5146b169ad6c/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/b4c8d360c7d4/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/02292d9fcbde/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/9e213b99c3d8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/1a3b5855e7fb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/faf65d286ef6/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/2703318c0308/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/fca2c9f58078/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/f6f75b38eaab/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/fc785f8b5088/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/f5d59f1f6413/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/683a/7016019/5146b169ad6c/gr10.jpg

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