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一种基于新型刺突（S）蛋白的商业免疫测定法检测抗SARS-CoV-2 IgG的评估

Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG.

作者信息

Eberhardt Kirsten Alexandra, Dewald Felix, Heger Eva, Gieselmann Lutz, Vanshylla Kanika, Wirtz Maike, Kleipass Franziska, Johannis Wibke, Schommers Philipp, Gruell Henning, Brensing Karl August, Müller Roman-Ulrich, Augustin Max, Lehmann Clara, Koch Manuel, Klein Florian, Di Cristanziano Veronica

机构信息

Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine & I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.

Institute for Transfusion Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.

出版信息

Microorganisms. 2021 Mar 31;9(4):733. doi: 10.3390/microorganisms9040733.

DOI:10.3390/microorganisms9040733
PMID:33807490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8067155/
Abstract

: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Overall, the IDK® anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. The novel IDK anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.

摘要

对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗体反应的研究是应对新型冠状病毒肺炎（COVID-19）大流行的一个关键方面。在本研究中，我们将新的免疫诊断公司（Immundiagnostik）IDK®抗SARS-CoV-2 S1 IgG检测方法与四种广泛使用的商业血清学检测方法进行了比较，以检测针对刺突（S）蛋白和核衣壳（NC）蛋白的抗体。血清样本取自一组无偏倚的康复患者和一个阴性对照组。样本同时通过新的免疫诊断公司IDK抗SARS-CoV-2 S1 IgG检测方法、索林集团（DiaSorin）LIAISON SARS-CoV-2 S1/S2 IgG检测方法以及欧蒙（Euroimmun）抗SARS-CoV-2 S1 IgG酶联免疫吸附测定（ELISA）进行分析。通过雅培（Abbott）SARS-CoV-2 IgG检测方法和罗氏（Roche）电化学发光免疫分析法（Elecsys）抗SARS-CoV-2全免疫球蛋白免疫测定法检测结合NC的抗体。此外，我们还对一组最初检测S1 IgG呈非反应性的COVID-19康复受试者的样本进行了研究。样本还通过活病毒和假病毒中和试验进行检测。总体而言，IDK®抗SARS-CoV-2 S1 IgG检测方法在评估的基于刺突（S）蛋白的检测方法中显示出最高的灵敏度。此外，免疫诊断检测方法与血清中和活性具有良好的相关性。新型IDK抗SARS-CoV-2 S1 IgG检测方法显示出高灵敏度和特异性，是用于常规诊断的一个有效选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/5f18d010e424/microorganisms-09-00733-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/6402acaf83a5/microorganisms-09-00733-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/40c1f9aeb775/microorganisms-09-00733-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/c4c6c8fdb389/microorganisms-09-00733-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/cf0d458ad822/microorganisms-09-00733-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/83487515943b/microorganisms-09-00733-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/5f18d010e424/microorganisms-09-00733-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/6402acaf83a5/microorganisms-09-00733-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/40c1f9aeb775/microorganisms-09-00733-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/c4c6c8fdb389/microorganisms-09-00733-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/cf0d458ad822/microorganisms-09-00733-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/83487515943b/microorganisms-09-00733-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d9b/8067155/5f18d010e424/microorganisms-09-00733-g006.jpg

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