Siddaiah Roopa, Oji-Mmuo Christiana N, Montes Deborah T, Fuentes Nathalie, Spear Debra, Donnelly Ann, Silveyra Patricia
Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Biobehavioral Laboratory, School of Nursing, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Biomedicines. 2021 Mar 5;9(3):257. doi: 10.3390/biomedicines9030257.
Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth, arrested fetal lung development, and inflammation. The incidence of BPD remains on the rise as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of BPD severity. We collected tracheal aspirates (TAs) from preterm babies with mild/moderate ( = 8) and severe ( = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, false discovery rate (FDR) < 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD group when compared to the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extremely premature infants contain miRNA signatures associated with severe BPD. These may serve as potential biomarkers of disease severity in infants with BPD.
支气管肺发育不良（BPD）是一种慢性肺部疾病，发生于新生儿，是早产、胎儿肺发育停滞和炎症的结果。由于极早产儿存活率的提高，BPD的发病率持续上升。严重的BPD导致高昂的医疗费用，并与住院时间延长、呼吸道感染和神经发育缺陷有关。在本研究中，我们旨在检测BPD严重程度的新型生物标志物。我们收集了患有轻度/中度（n = 8）和重度（n = 17）BPD的早产儿的气管吸出物（TA），并使用PCR阵列分析了1048种miRNA的表达。使用 Ingenuity Pathway Analysis（IPA）软件确定与生物途径的关联。我们发现两个疾病组之间有31种miRNA差异表达（变化倍数为2倍，错误发现率（FDR）< 0.05）。其中，与轻度/中度BPD组相比，重度BPD组中有4种miRNA表达水平显著升高，27种miRNA表达水平显著降低。IPA确定了与miRNA特征相关的细胞信号传导和炎症途径。我们得出结论，极早产儿的TA含有与严重BPD相关的miRNA特征。这些可能作为BPD婴儿疾病严重程度的潜在生物标志物。
