Department of Experimental Medicine, Luigi Vanvitelli Campania University, 80138 Naples, Italy.
Research Institute on Ecosystems (IRET), CNR, 80131 Naples, Italy.
Cells. 2021 Mar 30;10(4):761. doi: 10.3390/cells10040761.
The cells present in the stromal compartment of many tissues are a heterogeneous population containing stem cells, progenitor cells, fibroblasts, and other stromal cells. A SSEA3(+) cell subpopulation isolated from human stromal compartments showed stem cell properties. These cells, known as multilineage-differentiating stress-enduring (MUSE) cells, are capable of resisting stress and possess an excellent ability to repair DNA damage. We isolated MUSE cells from different mouse stromal compartments, such as those present in bone marrow, subcutaneous white adipose tissue, and ear connective tissue. These cells showed overlapping in vitro biological properties. The mouse MUSE cells were positive for stemness markers such as SOX2, OCT3/4, and NANOG. They also expressed TERT, the catalytic telomerase subunit. The mouse MUSE cells showed spontaneous commitment to differentiation in meso/ecto/endodermal derivatives. The demonstration that multilineage stem cells can be isolated from an animal model, such as the mouse, could offer a valid alternative to the use of other stem cells for disease studies and envisage of cellular therapies.
许多组织的基质隔室中存在的细胞是一个异质群体，其中包含干细胞、祖细胞、成纤维细胞和其他基质细胞。从人类基质隔室中分离出的 SSEA3(+)细胞亚群具有干细胞特性。这些细胞被称为多谱系分化应激耐受 (MUSE) 细胞，能够抵抗应激，并且具有出色的修复 DNA 损伤的能力。我们从不同的小鼠基质隔室中分离出 MUSE 细胞，例如骨髓、皮下白色脂肪组织和耳部结缔组织。这些细胞表现出重叠的体外生物学特性。小鼠 MUSE 细胞呈干细胞标志物阳性，如 SOX2、OCT3/4 和 NANOG。它们还表达 TERT，即端粒酶催化亚基。小鼠 MUSE 细胞表现出自发地向中胚层/外胚层/内胚层衍生物分化的趋势。证明多能干细胞可以从动物模型（如小鼠）中分离出来，这为使用其他干细胞进行疾病研究和细胞治疗提供了有效的替代方案。
