Segura Verónica, Díaz Jacobo, Ruiz-Carnicer Ángela, Muñoz-Suano Alba, Carrillo-Carrión Carolina, Sousa Carolina, Cebolla Ángel, Comino Isabel
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.
Clinical Analysis Service, Hospital Universitario INGESA, 51003 Ceuta, Spain.
Foods. 2021 Mar 19;10(3):652. doi: 10.3390/foods10030652.
One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA.
麸质分析中的一个主要关注点是实现麸质蛋白的高效提取。传统的基于乙醇的提取溶液效率低下，因此，需要使用还原剂或酸来实现适当的溶解。食品法典委员会标准118 - 1979（2008年修订）推荐的提取方法使用混合溶液（专利WO 02/092633 A1）。然而，它具有危害性且气味难闻，并且与一些免疫技术不兼容。在此，使用不同的方法条件、食品基质和各种免疫方法评估了一种新型通用麸质提取溶液（UGES）（专利ES 2 392 412 A1）的通用性和提取能力。UGES对使用者和环境而言都包含更安全的化合物，并且它显示出与夹心酶联免疫吸附测定（ELISA）推荐的提取方法相似的提取效率。根据样品类型，提取时间从100分钟显著减少至40分钟。此外，与目前使用的溶液不同，UGES与竞争性ELISA兼容。
