Pellegrini Marika, Spera Daniela M, Ercole Claudia, Del Gallo Maddalena
AGIRE Soc. Cons. a r.l., Via Isidoro e Lepido Facii, 64100 Teramo, Italy.
Department of Life, Health and Environmental Sciences, University of L'Aquila, Via Vetoio, Coppito, 67010 L'Aquila, Italy.
Microorganisms. 2021 Mar 19;9(3):639. doi: 10.3390/microorganisms9030639.
The present work was aimed at investigating the effects of a four bacterial strain consortium-, and -on L. and on soil health. The bacterial consortium was inoculated on seeds of two different onion varieties; inoculated and Control seeds (treated with autoclaved inoculum) were sown in an open-field and followed until harvest. Plant growth development parameters, as well as soil physico-chemical and molecular profiles (DNA extraction and 16S community sequencing on the Mi-Seq Illumina platform), were investigated. The results showed a positive influence of bacterial application on plant growth, with increased plant height (+18%), total chlorophylls (+42%), crop yields (+13%), and bulb dry matter (+3%) with respect to the Control. The differences between Control and treatments were also underlined in the bulb extracts in terms of total phenolic contents (+25%) and antioxidant activities (+20%). Soil fertility and microbial community structure and diversity were also positively affected by the bacterial inoculum. At harvest, the soil with the presence of the bacterial consortium showed an increase in total organic carbon, organic matter, and available phosphorus, as well as higher concentrations of nutrients than the Control. The ecological indexes calculated from the molecular profiles showed that community diversity was positively affected by the bacterial treatment. The present work showed the effective use of plant growth-promoting bacteria as a valid fertilization strategy to improve yield in productive landscapes whilst safeguarding soil biodiversity.
本研究旨在调查由四种菌株组成的菌剂对洋葱生长及土壤健康的影响。将该菌剂接种于两种不同洋葱品种的种子上；接种种子和对照种子（用经高压灭菌的接种物处理）在露天田地播种，并跟踪至收获。研究了植物生长发育参数以及土壤理化和分子特征（DNA提取以及在Mi-Seq Illumina平台上进行16S群落测序）。结果表明，与对照相比，施用菌剂对植物生长有积极影响，株高增加了18%，总叶绿素增加了42%，作物产量增加了13%，鳞茎干物质增加了3%。对照与处理之间的差异在鳞茎提取物的总酚含量（增加25%）和抗氧化活性（增加20%）方面也很明显。土壤肥力以及微生物群落结构和多样性也受到菌剂的积极影响。收获时，存在菌剂的土壤总有机碳、有机质和有效磷增加，且养分浓度高于对照。根据分子特征计算的生态指数表明，细菌处理对群落多样性有积极影响。本研究表明，有效利用促植物生长细菌作为一种有效的施肥策略，可在保障土壤生物多样性的同时提高生产性景观中的产量。
