Masia Maria Dolores, Dettori Marco, Deriu Grazia Maria, Bellu Sabina, Arcadu Lisa, Azara Antonio, Piana Andrea, Palmieri Alessandra, Arghittu Antonella, Castiglia Paolo
Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy.
University Hospital of Sassari, 07100 Sassari, Italy.
Healthcare (Basel). 2021 Mar 19;9(3):352. doi: 10.3390/healthcare9030352.
Achieving sterilization by adopting proper practices is essential to ensure that surgical instruments do not transmit microorganisms to patients. As the effectiveness of sterilization mandates effective cleaning, it is necessary to verify the success of cleaning procedures. In this study, we used the adenosine triphosphate (ATP) bioluminescence method for assessing the efficacy of the manual cleaning procedure during the reprocessing of reusable surgical instruments. The ATP bioluminescence assay was performed on 140 surgical instruments of 12 different types, both before being cleaned (baseline) and after each of the cleaning procedures (i.e., decontamination, manual washing, drying, and visual inspection). For each instrument, two swabs were used as follows: one to sample the entire surface (test point 1) and the other to sample the most difficult part of the surface to clean (test point 2). Overall, for each type of instrument, there was a decrease in contamination ranging from 99.6 to >99.9% (log reduction from 2.40 to 3.76). Thus, in order to standardize the assessment of cleanliness, it may be useful to introduce the bioluminescence method into the daily routine or, at least, at regular time intervals as a complementary check combined with visual inspection. This would allow real-time verification of the achievement of an adequate level of cleanliness.
采用适当的方法实现灭菌对于确保手术器械不将微生物传播给患者至关重要。由于灭菌效果要求有效的清洁，因此有必要验证清洁程序的成功与否。在本研究中，我们使用三磷酸腺苷（ATP）生物发光法评估可重复使用手术器械再处理过程中手工清洁程序的效果。对12种不同类型的140件手术器械在清洁前（基线）和每个清洁程序（即去污、手工清洗、干燥和目视检查）后进行ATP生物发光测定。对于每件器械，使用两个拭子如下：一个对整个表面进行采样（测试点1），另一个对表面最难清洁的部分进行采样（测试点2）。总体而言，对于每种类型的器械，污染减少范围为99.6%至>99.9%（对数减少从2.40至3.76）。因此，为了标准化清洁度评估，将生物发光法引入日常工作或至少定期作为与目视检查相结合的补充检查可能是有用的。这将允许实时验证是否达到了足够的清洁水平。
