Lenkowski Marcin, Nijakowski Kacper, Kaczmarek Mariusz, Surdacka Anna
Department of Conservative Dentistry and Endodontics, Poznan University of Medical Sciences, 60-812 Poznan, Poland.
Department of Cancer Immunology, Poznan University of Medical Sciences, 61-866 Poznan, Poland.
J Clin Med. 2021 Mar 12;10(6):1189. doi: 10.3390/jcm10061189.
The course of periodontal disease is affected by many factors; however, the most significant are the dysbiotic microflora, showing different pathogenicity levels. Rapid colonization in the subgingival environment can radically change the clinical state of the periodontium. This systematic review aims to present an innovative technique of loop-mediated isothermal amplification for rapid panel identification of bacteria in periodontal diseases. The decisive advantage of the loop-mediated isothermal amplification (LAMP) technique in relation to molecular methods based on the identification of nucleic acids (such as polymerase chain reaction (PCR or qPCR) is the ability to determine more pathogens simultaneously, as well as with higher sensitivity. In comparison with classical microbiological seeding techniques, the use of the LAMP method shortens a few days waiting time to a few minutes, reducing the time necessary to identify the species and determine the number of microorganisms. The LAMP technology requires only a small hardware base; hence it is possible to use it in outpatient settings. The developed technique provides the possibility of almost immediate assessment of periodontal status and, above all, risk assessment of complications during the treatment (uncontrolled spread of inflammation), which can certainly be of key importance in clinical work.
牙周病的病程受多种因素影响；然而，最重要的是生态失调的微生物群，其致病性水平各不相同。在龈下环境中的快速定植会从根本上改变牙周组织的临床状态。本系统评价旨在介绍一种用于快速鉴定牙周病中细菌的环介导等温扩增创新技术。与基于核酸鉴定的分子方法（如聚合酶链反应（PCR或qPCR））相比，环介导等温扩增（LAMP）技术的决定性优势在于能够同时检测更多病原体，且灵敏度更高。与传统的微生物接种技术相比，使用LAMP方法将几天的等待时间缩短至几分钟，减少了鉴定菌种和确定微生物数量所需的时间。LAMP技术仅需少量硬件设备；因此可以在门诊环境中使用。所开发的技术提供了几乎立即评估牙周状况的可能性，最重要的是，可以评估治疗期间并发症的风险（炎症的失控扩散），这在临床工作中肯定至关重要。
