Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Int J Mol Sci. 2021 Mar 12;22(6):2900. doi: 10.3390/ijms22062900.
Clematis plants play an important role in botanical gardens. Heat stress can destroy the activity, state and conformation of plant proteins, and its regulatory pathway has been well characterized in and some crop plants. However, the heat resistance response mechanism in horticultural plants including has rarely been reported. Here, we identified a heat-tolerant clematis species, . The relative water loss and electrolytic leakage were significantly lower under heat treatment in compared to Stolwijk Gold. Differential expression heat-tolerant genes (HTGs) were identified based on nonparametric transcriptome analysis. For validation, one heat shock transcription factor, , extremely induced by heat stimuli in , was identified to confer tolerance to heat stress in and . Furthermore, silencing of by virus-induced gene silencing (VIGS) led to heat sensitivity in tobacco and , suggesting that the candidate heat-resistant genes identified in this RNA-seq analysis are credible and offer significant utility. We also found that improved heat tolerance of by elevating heat shock protein (HSP) expression, which was negatively regulated by . Taken together, this study provides insights into the mechanism of heat tolerance and the findings can be potentially applied in horticultural plants to improve economic efficiency through genetic approaches.
铁线莲植物在植物园中起着重要作用。热应激会破坏植物蛋白质的活性、状态和构象，其调控途径在 和一些作物中得到了很好的描述。然而，包括 在内的园艺植物的耐热性响应机制很少有报道。在这里，我们鉴定了一个耐热的铁线莲种， 。与 Stolwijk Gold 相比， 在热处理下相对水分损失和电导率泄漏明显较低。基于非参数转录组分析，鉴定了差异表达的耐热基因（HTGs）。为了验证，一个热休克转录因子 ， 在 中受到热刺激的强烈诱导，被鉴定为在 和 中赋予耐热性。此外，通过病毒诱导的基因沉默（VIGS）沉默 导致烟草和 对热敏感，这表明从这个 RNA-seq 分析中鉴定出的候选耐热基因是可信的，具有重要的应用价值。我们还发现 通过提高热休克蛋白（HSP）的表达来提高 的耐热性，而 HSP 的表达受 负调控。总之，这项研究为 耐热性的机制提供了深入的了解，这些发现可以通过遗传方法应用于园艺植物，以提高经济效益。
