Pharmacology Research Laboratory, Department of Pharmacology, Khalsa College of Pharmacy, Amritsar, India.
Department of Pharmaceutical Analysis, Khalsa College of Pharmacy, Amritsar, India.
Burns Trauma. 2016 Mar 16;4:7. doi: 10.1186/s41038-016-0032-1. eCollection 2016.
The present study was designed to evaluate the potential of isoquercetin-based cream formulation on scald burn wound injury in rats.
Four isoquercetin-based cream formulations viz. 0.01, 0.02, 0.04, and 0.06 % w/w were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scald burn was given to rats by pouring water at 90 °C on a shaved dorsal area of 20 mm(2). Deep second-degree burn injury was produced which was evaluated for the next 21 days for the percentage of wound contraction and period of epithelialization. On day 21, the rats were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive species (TBARS) and reduced glutathione (GSH) estimation.
There was a significant increase in the percentage of wound contraction and a significant decrease in the period of epithelialization in isoquercetin-based cream-treated groups as compared with the control group. However, most significant results were obtained with isoquercetin 0.06 % w/w cream. Histologically, isoquercetin 0.06 % w/w cream treatment resulted in almost complete re-epithelialization and re-structuring of the wound tissue. There was a significant rise in TBARS and a decrease in GSH levels in the burn injury group which was reversed to a major extent by the application of isoquercetin-based cream.
The results indicate the wound healing potential of isoquercetin-based cream. Tissue biochemical studies indicate towards a possible role of free radical scavenging in the observed effects of isoquercetin in wound healing.
本研究旨在评估异槲皮苷乳膏制剂对大鼠烫伤创面损伤的潜在作用。
制备了四种异槲皮苷乳膏制剂，即 0.01%、0.02%、0.04%和 0.06%w/w。乳膏基质和含有磺胺嘧啶银的标准烧伤乳膏也被用于比较。通过将 90°C 的水倒在剃光的 20mm(2)背部区域上，给大鼠造成烫伤烧伤。产生深度二度烧伤损伤，在接下来的 21 天内评估伤口收缩百分比和上皮化时间。第 21 天，处死大鼠，用苏木精-伊红染色制备组织病理学切片。使用硫代巴比妥酸反应性物质 (TBARS) 和还原型谷胱甘肽 (GSH) 估计来筛选烧伤组织的氧化应激水平。
与对照组相比，异槲皮苷乳膏处理组的伤口收缩百分比显著增加，上皮化时间显著缩短。然而，异槲皮苷 0.06%w/w 乳膏的结果最为显著。组织学上，异槲皮苷 0.06%w/w 乳膏治疗导致伤口组织几乎完全再上皮化和重新结构。烧伤损伤组的 TBARS 水平显著升高，GSH 水平显著降低，而异槲皮苷乳膏的应用在很大程度上逆转了这一趋势。
结果表明异槲皮苷乳膏具有促进伤口愈合的潜力。组织生化研究表明，异槲皮苷在伤口愈合中的作用可能与其清除自由基有关。
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