Yadav Ashutosh Kumar, Kumar Aruna, Grover Nitasha, Ellur Ranjith Kumar, Bollinedi Haritha, Krishnan Subbaiyan Gopala, Bhowmick Prolay Kumar, Vinod Kunnummal Kurungara, Nagarajan Mariappan, Singh Ashok Kumar
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Amity Institute of Biotechnology, Amity University, Noida 201303, India.
Plants (Basel). 2021 Mar 16;10(3):559. doi: 10.3390/plants10030559.
Rice germplasm is a rich resource for discovering genes associated with salt tolerance. In the current study, a set of 96 accessions were evaluated for seedling stage salinity tolerance and its component traits. Significant phenotypic variation was observed among the genotypes for all the measured traits and eleven accessions with high level of salt tolerance at seedling stage were identified. The germplasm set comprised of three sub-populations and genome-wide association study (GWAS) identified a total of 23 marker-trait associations (MTAs) for traits studied. These MTAs were located on rice chromosomes 1, 2, 5, 6, 7, 9, and 12 and explained the trait phenotypic variances ranging from 13.98 to 29.88 %. Twenty-one MTAs identified in this study were located either in or near the previously reported quantitative trait loci (QTLs), while two MTAs namely, qSDW2.1 and qSNC5 were novel. A total of 18 and 13 putative annotated candidate genes were identified in a genomic region spanning ~200 kb around the MTAs and respectively. Some of the important genes underlying the novel MTAs were , and which are known to be associated with salinity tolerance in crops. These MTAs pave way for combining salinity tolerance with high yield in rice genotypes through molecular breeding.
水稻种质是发现与耐盐性相关基因的丰富资源。在本研究中，对一组96份种质进行了苗期耐盐性及其组成性状的评估。在所有测量性状的基因型之间观察到显著的表型变异，并鉴定出11份在苗期具有高耐盐水平的种质。该种质集由三个亚群组成，全基因组关联研究（GWAS）共鉴定出23个与所研究性状相关的标记-性状关联（MTA）。这些MTA位于水稻第1、2、5、6、7、9和12号染色体上，解释的性状表型变异范围为13.98%至29.88%。本研究中鉴定出的21个MTA位于先前报道的数量性状位点（QTL）内或附近，而两个MTA，即qSDW2.1和qSNC5是新发现的。在MTA周围约200 kb的基因组区域中分别鉴定出总共18个和13个推定的注释候选基因。新发现的MTA所涉及的一些重要基因是 ， ， ，这些基因已知与作物的耐盐性有关。这些MTA为通过分子育种将水稻基因型的耐盐性与高产相结合铺平了道路。
