Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Center of Excellence in Environment and Plant Physiology (CEEPP), Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Mol Sci. 2022 Feb 6;23(3):1842. doi: 10.3390/ijms23031842.
Salt stress is a major limiting factor in crop production and yield in many regions of the world. The objective of this study was to identify the genes responsible for salt tolerance in Thai rice populations. We performed a genome-wide association study with growth traits, relative water content, and cell membrane stability at the seedling stage, and predicted 25 putative genes. Eleven of them were located within previously reported salt-tolerant QTLs (ST-QTLs). , located outside the ST-QTLs, was selected for gene characterization using the mutant line with T-DNA insertion in the orthologous gene. Mutations in the gene led to the enhancement of salt tolerance by increasing the ability to maintain photosynthetic pigment content and relative water content, while the complemented lines with ectopic expression of showed more susceptibility to salt stress detected by photosynthesis performance. Moreover, the salt-tolerant rice varieties showed lower expression of this gene than the susceptible rice varieties under salt stress conditions. The study concludes that by acting as a negative regulator, OsCRN plays an important role in salt tolerance in rice.
盐胁迫是世界许多地区作物生产和产量的主要限制因素。本研究的目的是鉴定泰国水稻群体中耐盐相关的基因。我们对生长特性、幼苗期相对含水量和细胞膜稳定性进行了全基因组关联研究，预测了 25 个可能的基因。其中 11 个位于先前报道的耐盐 QTL（ST-QTL）内。位于 ST-QTL 之外的 基因，被选择用于使用 T-DNA 插入在同源基因中的 突变体系进行基因特征描述。该基因的突变导致通过增加维持光合色素含量和相对含水量的能力来增强耐盐性，而异位表达 的互补系表现出对盐胁迫更敏感，通过光合作用性能检测到。此外，耐盐水稻品种在盐胁迫条件下比敏感水稻品种表现出更低的基因表达。该研究得出结论，OsCRN 作为一个负调节剂，在水稻的耐盐性中起着重要作用。
