Musin Egor V, Kim Aleksandr L, Dubrovskii Alexey V, Kudryashova Ekaterina B, Ariskina Elena V, Tikhonenko Sergey A
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Institutskaya st. 3, 142290 Puschino, Russia.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prospect Nauki 5, 142290 Pushchino, Russia.
Polymers (Basel). 2021 Mar 16;13(6):914. doi: 10.3390/polym13060914.
Phage therapy is a great alternative to antibiotic drugs, but it can't effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival. The work studied the effect of polyanions and polycations on the coliprotetic bacteriophage's viability. We have shown that polyallylamine decreased bacteriophage's viability during increasing polyelectrolyte concentration and polyarginine had a lower inhibitory effect (then PAH) on the activity of the bacteriophage due to polyelectrolyte concentration from 0.05 to 5 mg/mL. It was shown that the inhibition of the bacteriophage by polyallylamine had an electrostatic nature and the use of high ionic strength prevented the formation of the PAH-protein capsid complex. Polystyrene sulfonate does not affect bacteriophage viability during increasing polyelectrolyte concentration from 0.05 mg/mL to 1 mg/mL. Polystyrene sulfonate decreases the viability of bacteriophage from 5 mg/mL of polyelectrolyte concentration. Dextran sulfate inhibits bacteriophage activity at 20-30%. Dextran inhibits bacteriophage activity by 80% at diapason concentration from 0.05 to 5 mg/mL and loses the inhibition effect from a concentration of 5 mg/mL.
噬菌体疗法是抗生素药物的一种很好的替代方法，但它无法有效克服胃酸过多的环境。我们提出使用聚电解质微胶囊作为噬菌体的一种保护手段。有必要了解聚电解质对噬菌体存活的影响。这项研究探讨了聚阴离子和聚阳离子对大肠杆菌噬菌体活力的影响。我们发现，随着聚电解质浓度的增加，聚烯丙胺会降低噬菌体的活力，而在聚电解质浓度从0.05毫克/毫升到5毫克/毫升的范围内，聚精氨酸对噬菌体活性的抑制作用较低（低于聚烯丙胺）。结果表明，聚烯丙胺对噬菌体的抑制具有静电性质，使用高离子强度可防止聚烯丙胺 - 蛋白质衣壳复合物的形成。在聚电解质浓度从0.05毫克/毫升增加到1毫克/毫升的过程中，聚苯乙烯磺酸盐不会影响噬菌体的活力。当聚电解质浓度达到5毫克/毫升时，聚苯乙烯磺酸盐会降低噬菌体的活力。硫酸葡聚糖在20% - 30% 的范围内抑制噬菌体活性。在浓度范围从0.05毫克/毫升到5毫克/毫升时，葡聚糖对噬菌体活性的抑制率为80% ，当浓度达到5毫克/毫升时失去抑制作用。
