Department of Swine Diseases, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100 Pulawy, Poland.
Department of Anatomopathology, National Veterinary Research Institute, Partyzantów 57 Avenue, 24-100 Pulawy, Poland.
Viruses. 2021 Mar 22;13(3):521. doi: 10.3390/v13030521.
This study aimed to indicate the influence of infection caused by genotype II African swine fever virus (ASFV)-isolate Pol18_28298_O111, currently circulating in Poland, on blood counts, biochemical parameters, as well as inflammatory and immune responses. Blood and sera collected from 21 domestic pigs infected intranasally with different doses of virulent ASFV were analysed. The infection led to variable changes in blood counts depending on the stage of the disease with a tendency towards leukopenia and thrombocytopenia. The elevated C-reactive protein (CRP) concentrations and microscopic lesions in organs confirmed the development of the inflammation process, which also resulted in an increased level of biochemical markers such as: Aspartate transaminase (AST), creatine kinase (CK), creatinine, and urea. Antibodies could be detected from 9 to 18 days post infection (dpi). Two survivors presented the highest titer of antibodies (>5 log/mL) with a simultaneous increase in the lymphocyte T (CD3+) percentage-revealed by flow cytometry. Results confirmed a progressive inflammatory process occurring during the ASFV infection, which may lead to multiple organs failure and death of the majority of affected animals.
本研究旨在表明目前在波兰流行的基因型 II 非洲猪瘟病毒（ASFV）-分离株 Pol18_28298_O111 感染对血液计数、生化参数以及炎症和免疫反应的影响。从 21 头经鼻腔感染不同剂量强毒 ASFV 的家猪中采集血液和血清进行分析。感染导致血液计数发生变化，具体取决于疾病阶段，表现为白细胞减少和血小板减少的趋势。C 反应蛋白（CRP）浓度升高和器官的显微镜病变证实了炎症过程的发展，这也导致了生化标志物如天冬氨酸转氨酶（AST）、肌酸激酶（CK）、肌酐和尿素的水平升高。感染后 9 至 18 天可检测到抗体。两名幸存者表现出最高的抗体滴度（>5 对数/ml），同时通过流式细胞术显示淋巴细胞 T（CD3+）百分比增加。结果证实了 ASFV 感染过程中发生的进行性炎症过程，这可能导致多个器官衰竭和大多数受感染动物的死亡。
