一株七基因缺失的非洲猪瘟病毒可作为减毒活疫苗在猪中安全有效。
A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs.
机构信息
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, 150069, China.
出版信息
Sci China Life Sci. 2020 May;63(5):623-634. doi: 10.1007/s11427-020-1657-9. Epub 2020 Mar 1.
Abstract
African swine fever (ASF) is a devastating infectious disease in swine that is severely threatening the global pig industry. An efficacious vaccine is urgently required. Here, we used the Chinese ASFV HLJ/18 as a backbone and generated a series of gene-deleted viruses. The virulence, immunogenicity, safety, and protective efficacy evaluation in specific-pathogen-free pigs, commercial pigs, and pregnant sows indicated that one virus, namely HLJ/18-7GD, which has seven genes deleted, is fully attenuated in pigs, cannot convert to the virulent strain, and provides complete protection of pigs against lethal ASFV challenge. Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV, and as such is expected to play an important role in controlling the spread of ASFV.
摘要
非洲猪瘟（ASF）是一种严重威胁全球养猪业的猪的毁灭性传染病。迫切需要一种有效的疫苗。在这里，我们使用中国 ASFV HLJ/18 作为骨架，生成了一系列基因缺失病毒。在 SPF 猪、商业猪和妊娠母猪中的毒力、免疫原性、安全性和保护效力评估表明，一种具有七个基因缺失的病毒，即 HLJ/18-7GD，在猪中完全减毒，不能转化为强毒株，并能为猪提供针对致命 ASF 挑战的完全保护。我们的研究表明，HLJ/-18-7GD 是一种安全有效的 ASF 疫苗，有望在控制 ASF 的传播中发挥重要作用。
相似文献
1
A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs.一株七基因缺失的非洲猪瘟病毒可作为减毒活疫苗在猪中安全有效。
Sci China Life Sci. 2020 May;63(5):623-634. doi: 10.1007/s11427-020-1657-9. Epub 2020 Mar 1.
2
African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus.携带MGF360和MGF505基因缺失的非洲猪瘟病毒格鲁吉亚分离株在猪中减毒，并能抵御强毒亲本病毒的攻击。
J Virol. 2015 Jun;89(11):6048-56. doi: 10.1128/JVI.00554-15. Epub 2015 Mar 25.
3
Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge.从非洲猪瘟病毒格鲁吉亚2007分离株中同时删除9GL和UK基因可提高安全性并抵御同源攻击。
J Virol. 2016 Dec 16;91(1). doi: 10.1128/JVI.01760-16. Print 2017 Jan 1.
4
African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge.删除毒力相关基因9GL（B119L）的2007年格鲁吉亚株非洲猪瘟病毒，低剂量接种时可使猪只体内病毒减毒，并诱导产生针对同源攻击的有效保护。
J Virol. 2015 Aug;89(16):8556-66. doi: 10.1128/JVI.00969-15. Epub 2015 Jun 10.
5
Evaluation of an I177L gene-based five-gene-deleted African swine fever virus as a live attenuated vaccine in pigs.评估基于 I177L 基因缺失的非洲猪瘟病毒五个基因缺失株作为猪的活疫苗。
Emerg Microbes Infect. 2023 Dec;12(1):2148560. doi: 10.1080/22221751.2022.2148560.
6
Development of a Highly Effective African Swine Fever Virus Vaccine by Deletion of the I177L Gene Results in Sterile Immunity against the Current Epidemic Eurasia Strain.通过缺失I177L基因开发高效非洲猪瘟病毒疫苗可产生针对当前流行的欧亚毒株的无菌免疫。
J Virol. 2020 Mar 17;94(7). doi: 10.1128/JVI.02017-19.
7
BA71ΔCD2: a New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities.BA71ΔCD2：一种具有交叉保护能力的新型重组减毒非洲猪瘟病毒
J Virol. 2017 Oct 13;91(21). doi: 10.1128/JVI.01058-17. Print 2017 Nov 1.
8
Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate.建立一种 ELISA 方法以区分感染野型非洲猪瘟病毒和弱毒 HLJ/18-7GD 疫苗候选株的动物。
Viruses. 2022 Aug 6;14(8):1731. doi: 10.3390/v14081731.
9
Deletion of , , and Attenuates African Swine Fever Virus, and the Mutant Strain Confers Complete Protection against Homologous Challenges in Pigs.缺失 、 、 和 可减弱非洲猪瘟病毒的感染力，且该突变株可使猪完全免受同源性攻毒的影响。
J Virol. 2023 Apr 27;97(4):e0024723. doi: 10.1128/jvi.00247-23. Epub 2023 Apr 5.
10
African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge.携带9GL和MGF360/505基因缺失的非洲猪瘟病毒格鲁吉亚分离株在猪体内高度减毒，但不能抵御亲本病毒的攻击。
Virus Res. 2016 Aug 2;221:8-14. doi: 10.1016/j.virusres.2016.05.014. Epub 2016 May 12.
引用本文的文献
1
Evaluation of Cross-Protection of African Swine Fever Vaccine ASFV-G-ΔI177L Between ASFV Biotypes.非洲猪瘟疫苗ASFV-G-ΔI177L在不同生物型非洲猪瘟病毒间的交叉保护评估
Vaccines (Basel). 2025 Aug 13;13(8):858. doi: 10.3390/vaccines13080858.
2
Construction and Evaluation of Quadruple-Gene-Deleted Pseudorabies Virus Platforms for ASFV Antigen Delivery.用于非洲猪瘟病毒抗原递送的四基因缺失伪狂犬病病毒平台的构建与评价
Transbound Emerg Dis. 2025 Jul 16;2025:3628600. doi: 10.1155/tbed/3628600. eCollection 2025.
3
The Potential of Disabled Infectious Single Cycle (DISC) Virus Platforms for Next Generation African Swine Fever Vaccine Development.失活感染性单周期（DISC）病毒平台在下一代非洲猪瘟疫苗研发中的潜力
Transbound Emerg Dis. 2025 Jul 14;2025:8573171. doi: 10.1155/tbed/8573171. eCollection 2025.
4
African swine fever virus: Virology, pathogenesis, clinical impact, and global control strategies.非洲猪瘟病毒：病毒学、发病机制、临床影响及全球防控策略
Vet World. 2025 Jun;18(6):1599-1613. doi: 10.14202/vetworld.2025.1599-1613. Epub 2025 Jun 16.
5
Biomimetic phosphorus dendrimer multi-epitope nanovaccine enhances humoral and cellular immune response against African swine fever virus.仿生磷树枝状大分子多表位纳米疫苗增强针对非洲猪瘟病毒的体液免疫和细胞免疫反应。
J Nanobiotechnology. 2025 Jul 21;23(1):530. doi: 10.1186/s12951-025-03593-7.
6
Agricultural biotechnology in China: product development, commercialization, and perspectives.中国的农业生物技术：产品开发、商业化及前景
aBIOTECH. 2025 May 15;6(2):284-310. doi: 10.1007/s42994-025-00209-4. eCollection 2025 Jun.
7
A Multiepitope Nanovaccine Candidate Adjuvanted with Porcine Ferritin Scaffold for African Swine Fever Virus.一种以猪铁蛋白支架为佐剂的非洲猪瘟病毒多表位纳米疫苗候选物。
Vaccines (Basel). 2025 May 30;13(6):585. doi: 10.3390/vaccines13060585.
8
The Multigene Family Genes-Encoded Proteins of African Swine Fever Virus: Roles in Evolution, Cell Tropism, Immune Evasion, and Pathogenesis.非洲猪瘟病毒的多基因家族基因编码蛋白：在进化、细胞嗜性、免疫逃逸和发病机制中的作用
Viruses. 2025 Jun 19;17(6):865. doi: 10.3390/v17060865.
9
Deletion of the African Swine Fever Virus Gen in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs.删除格鲁吉亚2010分离株基因组中的非洲猪瘟病毒基因对家猪体内病毒复制或毒力无影响。
Viruses. 2025 Apr 23;17(5):603. doi: 10.3390/v17050603.
10
Dynamics of Serological and Mucosal Antibody Responses against African Swine Fever Viruses in Experimentally Infected Pigs.实验感染猪中针对非洲猪瘟病毒的血清学和黏膜抗体反应动态
Transbound Emerg Dis. 2023 Feb 25;2023:9959847. doi: 10.1155/2023/9959847. eCollection 2023.
本文引用的文献
1
Development of a Highly Effective African Swine Fever Virus Vaccine by Deletion of the I177L Gene Results in Sterile Immunity against the Current Epidemic Eurasia Strain.通过缺失I177L基因开发高效非洲猪瘟病毒疫苗可产生针对当前流行的欧亚毒株的无菌免疫。
J Virol. 2020 Mar 17;94(7). doi: 10.1128/JVI.02017-19.
2
Outbreak of African swine fever in South Korea, 2019.2019 年韩国非洲猪瘟疫情暴发。
Transbound Emerg Dis. 2020 Mar;67(2):473-475. doi: 10.1111/tbed.13483. Epub 2020 Jan 28.
3
Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.从非洲猪瘟病毒格鲁吉亚株基因组中删除 CD2 样基因不会使病毒在猪中减毒。
Sci Rep. 2020 Jan 16;10(1):494. doi: 10.1038/s41598-020-57455-3.
4
Architecture of African swine fever virus and implications for viral assembly.非洲猪瘟病毒的结构及其对病毒组装的影响。
Science. 2019 Nov 1;366(6465):640-644. doi: 10.1126/science.aaz1439. Epub 2019 Oct 17.
5
Outbreak of African Swine Fever, Vietnam, 2019.2019 年越南非洲猪瘟疫情爆发。
Emerg Infect Dis. 2019 Jul;25(7):1433-1435. doi: 10.3201/eid2507.190303. Epub 2019 Jul 17.
6
Genetic characterization of African swine fever virus in Cameroon, 2010-2018.2010-2018 年喀麦隆非洲猪瘟病毒的遗传特征。
J Microbiol. 2019 Apr;57(4):316-324. doi: 10.1007/s12275-019-8457-4. Epub 2019 Mar 30.
7
Development of vaccines against African swine fever virus.非洲猪瘟病毒疫苗的研制。
Virus Res. 2019 May;265:150-155. doi: 10.1016/j.virusres.2019.03.022. Epub 2019 Mar 25.
8
Replication and virulence in pigs of the first African swine fever virus isolated in China.中国分离的首例非洲猪瘟病毒在猪体内的复制与毒力。
Emerg Microbes Infect. 2019;8(1):438-447. doi: 10.1080/22221751.2019.1590128.
9
Genome sequences derived from pig and dried blood pig feed samples provide important insights into the transmission of African swine fever virus in China in 2018.来自猪和干血猪饲料样本的基因组序列为2018年非洲猪瘟病毒在中国的传播提供了重要见解。
Emerg Microbes Infect. 2019;8(1):303-306. doi: 10.1080/22221751.2019.1565915.
10
DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain.DNA-蛋白质疫苗接种策略无法抵御非洲猪瘟病毒亚美尼亚2007毒株的攻击。
Vaccines (Basel). 2019 Jan 28;7(1):12. doi: 10.3390/vaccines7010012.
Zotero 插件