State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China.
National High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, 150069, China.
Sci China Life Sci. 2020 May;63(5):623-634. doi: 10.1007/s11427-020-1657-9. Epub 2020 Mar 1.
African swine fever (ASF) is a devastating infectious disease in swine that is severely threatening the global pig industry. An efficacious vaccine is urgently required. Here, we used the Chinese ASFV HLJ/18 as a backbone and generated a series of gene-deleted viruses. The virulence, immunogenicity, safety, and protective efficacy evaluation in specific-pathogen-free pigs, commercial pigs, and pregnant sows indicated that one virus, namely HLJ/18-7GD, which has seven genes deleted, is fully attenuated in pigs, cannot convert to the virulent strain, and provides complete protection of pigs against lethal ASFV challenge. Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV, and as such is expected to play an important role in controlling the spread of ASFV.
非洲猪瘟（ASF）是一种严重威胁全球养猪业的猪的毁灭性传染病。迫切需要一种有效的疫苗。在这里，我们使用中国 ASFV HLJ/18 作为骨架，生成了一系列基因缺失病毒。在 SPF 猪、商业猪和妊娠母猪中的毒力、免疫原性、安全性和保护效力评估表明，一种具有七个基因缺失的病毒，即 HLJ/18-7GD，在猪中完全减毒，不能转化为强毒株，并能为猪提供针对致命 ASF 挑战的完全保护。我们的研究表明，HLJ/-18-7GD 是一种安全有效的 ASF 疫苗，有望在控制 ASF 的传播中发挥重要作用。
