Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
IT & Quality Unit, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Molecules. 2021 Mar 26;26(7):1876. doi: 10.3390/molecules26071876.
Parthenolide, a strong cytotoxic compound found in different parts of which motivated the authors to develop an optimized microwave-assisted extraction (MEA) method using Box-Behnken design (BBD) for efficient extraction of parthenolide from the stem of and its validation by high-performance thin-layer chromatography (HPTLC) and cytotoxic analysis. The optimized parameters for microwave extraction were determined as: 51.5 °C extraction temperature, 50.8 min extraction time, and 211 W microwave power. A quadratic polynomial model was found the most suitable model with of 0.9989 and coefficient of variation (CV) of 0.2898%. The high values of adjusted (0.9974), predicted (0.9945), and signal-to-noise ratio (74.23) indicated a good correlation and adequate signal, respectively. HPTLC analyzed the parthenolide (R = 0.16) content in methanol extract (TCME) at λ = 575 nm and found it as 0.9273% ± 0.0487% /, which was a higher than expected yield (0.9157% /). The TCME exhibited good cytotoxicity against HepG2 and MCF-7 cell lines (IC = 30.87 and 35.41 µg/mL, respectively), which further supported our findings of high parthenolide content in TCME. This optimized MAE method can be further applied to efficiently extract parthenolide from marketed herbal supplements containing different species.
小白菊内酯是一种存在于 不同部位的强细胞毒性化合物，这促使作者开发了一种使用 Box-Behnken 设计 (BBD) 的优化微波辅助提取 (MEA) 方法，以从 茎中高效提取小白菊内酯，并通过高效薄层色谱 (HPTLC) 和细胞毒性分析进行验证。微波提取的优化参数确定为：提取温度 51.5°C、提取时间 50.8 分钟和微波功率 211 W。发现二次多项式模型是最合适的模型，其 为 0.9989，变异系数 (CV) 为 0.2898%。调整后的 （0.9974）、预测的 （0.9945）和信噪比（74.23）的值较高，表明相关性良好，信号充足。HPTLC 在 575nm 处分析 甲醇提取物（TCME）中的小白菊内酯（R = 0.16）含量，并发现其含量为 0.9273% ± 0.0487% / ，这是高于预期的收率（0.9157% / ）。TCME 对 HepG2 和 MCF-7 细胞系表现出良好的细胞毒性（IC = 30.87 和 35.41µg/mL，分别），这进一步支持了我们在 TCME 中发现高小白菊内酯含量的研究结果。这种优化的 MAE 方法可以进一步应用于从含有不同 种的市售草药补充剂中高效提取小白菊内酯。
