Sun Hui, Ding Weina, Duan Wanying, Zhou Jie, Guo Lanping
School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
State Key Laboratory of Dao-Di Herbs, National Resource Center for Chinese Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Planta. 2021 Apr 3;253(5):87. doi: 10.1007/s00425-021-03619-y.
The proteins related to the biosynthesis of salvianolic acids and lignins were regulated by smoke-water and karrikinolide in Salvia miltiorrhiza hairy roots. The effects of smoke-water (SW) and karrikinolide (KAR) on the biosynthesis of salvianolic acids and lignins in Salvia miltiorrhiza hairy roots have been studied using proteomic technology. The results showed that a total of 1290 and 1678 differentially expressed proteins were respectively obtained in SW and KAR comparing to the control. Bioinformatics analysis indicated the differentially expressed proteins responding to SW and KAR treatments mainly involved in macromolecule metabolic process, cell part, binding, etc., and most of the proteins were located at the cytoplasm and cell membrane, followed by nuclear. In addition, the proteins involved in salvianolic acids biosynthesis were up-regulated, including 4-coumarate-CoA ligase (EC 6.2.1.12) and shikimate O-hydroxycinnamoyl-transferase (EC 2.3.1.133). Enzymes involved in lignins biosynthesis were also identified, e.g. cinnamyl-alcohol dehydrogenase (EC 1.1.1.195) and peroxidase (EC 1.11.1.7). The results indicated that proteins related to the biosynthesis of salvianolic acids and lignins were regulated by SW and KAR in S. miltiorrhiza hairy roots. This study will enhance our understanding of the mechanism by which SW and KAR on the biosynthesis of salvianolic acids and lignins in S. miltiorrhiza hairy roots.
丹参毛状根中与丹酚酸和木质素生物合成相关的蛋白质受烟水和独脚金内酯调控。利用蛋白质组学技术研究了烟水（SW）和独脚金内酯（KAR）对丹参毛状根中丹酚酸和木质素生物合成的影响。结果表明，与对照相比，SW和KAR处理分别获得了1290个和1678个差异表达蛋白。生物信息学分析表明，响应SW和KAR处理的差异表达蛋白主要参与大分子代谢过程、细胞部分、结合等，且大多数蛋白位于细胞质和细胞膜，其次是细胞核。此外，参与丹酚酸生物合成的蛋白上调，包括4-香豆酸-CoA连接酶（EC 6.2.1.12）和莽草酸O-羟基肉桂酰转移酶（EC 2.3.1.133）。还鉴定出了参与木质素生物合成的酶，如肉桂醇脱氢酶（EC 1.1.1.195）和过氧化物酶（EC 1.11.1.7）。结果表明，丹参毛状根中与丹酚酸和木质素生物合成相关的蛋白质受SW和KAR调控。本研究将增进我们对SW和KAR影响丹参毛状根中丹酚酸和木质素生物合成机制的理解。
