Departamento de Ciencias Básicas, Universidad Autónoma Metropolitana Azcapotzalco, Av. San Pablo 180, Col. Reynosa Tamaulipas, Azcapotzalco, Ciudad de México 02200, Mexico; Instituto de Física, Universidad Nacional Autónoma de México, Circuito de la Investigación Científica Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico.
Departamento de Ciencias Básicas, Universidad Autónoma Metropolitana Azcapotzalco, Av. San Pablo 180, Col. Reynosa Tamaulipas, Azcapotzalco, Ciudad de México 02200, Mexico.
Mater Sci Eng C Mater Biol Appl. 2021 Apr;123:111934. doi: 10.1016/j.msec.2021.111934. Epub 2021 Feb 3.
In the present study, graphite oxide, graphite oxide doped with 0.8 g AgNO named GrO-0.5Ag, and graphite oxide doped with 1.25 g AgNO named GrO-1.0Ag (silver-doped graphitic materials) were synthesized by the modified Hummers and chemical doping methods. Subsequently, its antimicrobial activity against Bacillus subtilis, Candida albicans, Escherichia coli, and Staphylococcus aureus microorganisms was evaluated by agar well diffusion test with 1 and 2 mg·ml of material concentrations. The silver-doped graphitic materials were characterized by X-Ray Diffraction, Fourier-Transform Infrared, Raman, X-Ray Photoelectron spectroscopies, and Scanning Electron Microscopy. The GrO-0.5Ag material showed a percentage of inhibition effect of 86, 82, 48, and 45% with respect to the positive control for Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans microorganisms, respectively. Otherwise, the GrO-1.0Ag material showed a percentage of inhibition effect of 54% with respect to the positive control for Bacillus subtilis and less than 50% for the other microorganisms. The material concentrations showed a slight change in the percentage of inhibition effect for all microorganisms. Graphite oxide functional groups, small crystal size, free silver ions, and silver carbonate formation in silver-doped graphitic materials presented a viable alternative to inhibit bacterial growth.
在本研究中，通过改良的 Hummers 法和化学掺杂法合成了氧化石墨、掺杂 0.8 g AgNO 的氧化石墨（命名为 GrO-0.5Ag）和掺杂 1.25 g AgNO 的氧化石墨（命名为 GrO-1.0Ag，即银掺杂石墨材料）。随后，通过琼脂孔扩散试验，以 1 和 2 mg·ml 的材料浓度，评估了其对枯草芽孢杆菌、白色念珠菌、大肠杆菌和金黄色葡萄球菌微生物的抗菌活性。采用 X 射线衍射、傅里叶变换红外、拉曼、X 射线光电子能谱和扫描电子显微镜对银掺杂石墨材料进行了表征。与阳性对照相比，GrO-0.5Ag 材料对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和白色念珠菌微生物的抑制率分别为 86%、82%、48%和 45%。相比之下，GrO-1.0Ag 材料对枯草芽孢杆菌的抑制率为 54%，对其他微生物的抑制率低于 50%。材料浓度对所有微生物的抑制率略有变化。银掺杂石墨材料中的氧化石墨官能团、小晶体尺寸、游离银离子和碳酸银的形成提供了一种可行的替代方法来抑制细菌生长。
