Department of Surgery and Leiden Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
Laboratory for Myology, Faculty of Behavioural and Movement Sciences, Amsterdam Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Mitochondrion. 2021 Jul;59:83-95. doi: 10.1016/j.mito.2021.03.014. Epub 2021 Apr 1.
Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.
代谢重编程和线粒体功能障碍是广泛的生理和病理过程的核心要素。虽然细胞培养已被确立为阐述生理学和疾病的多功能技术，但使用标准细胞培养方案研究代谢时，会受到 Crabtree 效应的严重干扰。该现象是指培养细胞适应于富含葡萄糖的培养基中的糖酵解表型，而远离氧化磷酸化，这使得细胞培养在某些研究领域的适用性受到质疑。在本系统评价中，我们旨在提供已报道的规避 Crabtree 效应的策略的全面概述和批判性评估。
