Li Hongwei, Yu Yanxue, Zhang Jian, Wang Yuhan, Zhang Liu, Zhai Junfeng, Zhang Yongjiang
Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China.
CAIQ Center for Biosafety in Sanya, Sanya, China.
Front Microbiol. 2023 Jun 28;14:1197700. doi: 10.3389/fmicb.2023.1197700. eCollection 2023.
The Colorado potato beetle (CPB) is an internationally recognized plant quarantine pest that causes serious losses to potato agricultural production. The gut microbiota plays an important role in its growth and development, and the olfactory system plays an important role in insect feeding behavior. The gut microbiota is known to be capable of inducing changes in the olfactory systems of insects. However, the way these associated gut microbes influence the feeding-related behaviors of CPBs remains unclear. To explore the relationship between them, fresh potato leaves immersed in a mixture of five antibiotics (tetracycline, penicillin, ofloxacin, ciprofloxacin, and ampicillin) at specific concentrations for 1 h were fed to adult CPBs to reduce the abundance of gut microbes. We found that the feeding behavior of CPBs was significantly affected by the gut microbiota and that was significantly higher in abundance in the control group than in the antibiotic group. We then used transcriptome sequencing to explore the differences in olfactory receptor genes in the heads of non-treatment and antibiotic-fed CPBs. Through Illumina Hiseq™ sequencing and screening of differential genes, we found that the olfactory receptor gene LdecOR9 was significantly upregulated and LdecOR17 was significantly downregulated after antibiotic feeding. A real-time polymerase chain reaction was used to verify the changes in olfactory receptor gene expression in the non-treatment groups and antibiotic-treated groups. The feeding behavior was partially rescued after CPBs were re-fed with intestinal bacteria. These results indicate that a certain amount of gut microbiota can result in the loss of the olfactory discrimination ability of CPBs to host plants. In summary, this study investigated the relationship between gut microbiota and olfactory genes, providing a reference for research on microbial control.
科罗拉多马铃薯甲虫（CPB）是一种国际公认的植物检疫性害虫，给马铃薯农业生产造成严重损失。肠道微生物群在其生长发育中起重要作用，嗅觉系统在昆虫取食行为中起重要作用。已知肠道微生物群能够诱导昆虫嗅觉系统发生变化。然而，这些相关的肠道微生物影响CPB取食相关行为的方式仍不清楚。为了探究它们之间的关系，将新鲜马铃薯叶片浸泡在特定浓度的五种抗生素（四环素、青霉素、氧氟沙星、环丙沙星和氨苄青霉素）混合液中1小时后，喂食给成年CPB，以降低肠道微生物的丰度。我们发现CPB的取食行为受到肠道微生物群的显著影响，对照组中其丰度显著高于抗生素组。然后，我们使用转录组测序来探究未处理和经抗生素处理的CPB头部嗅觉受体基因的差异。通过Illumina Hiseq™测序和差异基因筛选，我们发现抗生素处理后嗅觉受体基因LdecOR9显著上调，LdecOR17显著下调。使用实时聚合酶链反应来验证未处理组和抗生素处理组中嗅觉受体基因表达的变化。在用肠道细菌重新喂食CPB后，其取食行为部分得到恢复。这些结果表明，一定数量的肠道微生物群会导致CPB对寄主植物嗅觉辨别能力的丧失。综上所述，本研究调查了肠道微生物群与嗅觉基因之间的关系，为微生物防治研究提供了参考。
