Xu Dehui, Ning Ning, Xu Yujing, Xia Wenjie, Liu Dingxin, Chen Hailan, Kong Michael G
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an, China.
The School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.
Front Oncol. 2021 Mar 17;11:552480. doi: 10.3389/fonc.2021.552480. eCollection 2021.
Cold atmospheric plasma, including plasma jet and surface plasma, can promote the apoptosis of cancer cells without causing significant damage to surrounding normal cells, which was hopeful to be applied to the clinical cancer therapy. However, experimental plasma devices used directly to clinical experiments has challenges in technology and methods, especially the difference in killing tumor cells efficiency of these two common plasma sources. Therefore, it is great necessity to explore the differences in treating tumors between different plasma sources. This paper achieved good killing efficiency by using two kinds of cold atmospheric plasma generating devices, namely plasma jet and surface plasma treatment along acute myeloid leukemia (AML). The results showed that the He plasma jet kills leukemia cells more efficiently than surface plasma with the same voltage and frequency and the same time. By GC-TOFMS and metabolomics analysis, this paper compared the differential metabolites of leukemia cells treated by two plasma devices and the key metabolic pathways closely related to differential metabolites. Simultaneously, we found alanine, aspartate and glutamate metabolism was most correlated with a key differential metabolite, glutamine. It was found that the glutaminase activity of He plasma jet group was lower than that of surface plasma group, which might be a reason for He plasma jet group to kill tumor cells better. It was also worth noting that relative quantity of glucose metabolites of plasma jet treatment group was lower than that of surface plasma treatment group. This study provides the basis for clinical trials for future.
冷大气等离子体，包括等离子体射流和表面等离子体，可促进癌细胞凋亡，而不会对周围正常细胞造成显著损伤，有望应用于临床癌症治疗。然而，直接用于临床试验的实验性等离子体设备在技术和方法上存在挑战，尤其是这两种常见等离子体源在杀死肿瘤细胞效率方面存在差异。因此，探索不同等离子体源在治疗肿瘤方面的差异非常必要。本文通过使用两种冷大气等离子体发生装置，即等离子体射流和表面等离子体处理急性髓系白血病（AML），取得了良好的杀伤效率。结果表明，在相同电压、频率和时间下，氦等离子体射流比表面等离子体更有效地杀死白血病细胞。通过气相色谱-飞行时间质谱（GC-TOFMS）和代谢组学分析，本文比较了两种等离子体装置处理的白血病细胞的差异代谢物以及与差异代谢物密切相关的关键代谢途径。同时，我们发现丙氨酸、天冬氨酸和谷氨酸代谢与一种关键差异代谢物谷氨酰胺的相关性最高。结果发现，氦等离子体射流组的谷氨酰胺酶活性低于表面等离子体组，这可能是氦等离子体射流组更好地杀死肿瘤细胞的一个原因。还值得注意的是，等离子体射流处理组的葡萄糖代谢物相对含量低于表面等离子体处理组。本研究为未来的临床试验提供了依据。
