Fatemi Mohammad Javad, Garahgheshlagh Soheila Naderi, Ghadimi Tayyeb, Jamili Shahla, Nourani Mohammad Reza, Sharifi Ali Mohammad, Saberi Mohsen, Amini Naser, Sarmadi Vahid Hosseinpour, Yazdi-Amirkhiz Seyed Yasin
Burn Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Plastic and Reconstructive Surgery, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Tehran, Iran.
Regen Ther. 2021 Mar 18;18:12-20. doi: 10.1016/j.reth.2021.03.001. eCollection 2021 Dec.
The present study focused on burning as one of the main causes of mortality with detrimental economic and social effects in the world. The purpose of this study was to investigate the impact of collagen-chitosan gel extracted from and shrimp skin in the treatment of second degree burn healing among rats.
MATERIALS & METHOD: To fulfill the purpose of the study, chitosan and collagen were extracted respectively from shrimp and skin waste by the acid-based method and were evaluated by using Pico Tag, SDS-PAGE. The burn wound healing efficiency of marine collagen-chitosan gel was examined in vivo using rats. Three different ratios of collagen and chitosan blend (Col-CH, 1:3, 1:1 and 3:1) were prepared to obtain the most effective Col-CH gel for burn wound healing and were compared to the animals treated with silver sulfadiazine ointment. Healing burn wound was studied by measuring wound surface area with Image J and histopathologic examination was carried out based on the mean of epithelialization, fibroblastic cells, acute and chronic inflammatory cells, angiogenesis, structure collagen and the amount of collagen on days 15 and 25 post-burn.
The results of SDS-PAGE indicated that the extracted collagen was type I and it was composed of two α (α and α) chains. Amino acid analysis showed a much higher glaycin content in extracted collagen which amounted to one-third of the total amino. The wound surface measurement showed a significant reduction in wound size in the group treated with Col-CH (3:1) compared to silver-sulfadiazine treated group on 15th and 25th days. Histopathological findings represented a high score in epithelialization, collagen, collagen structure, fibroblast cell and a decrease in inflammatory cells infiltration in Col-CH (3:1) treated group on 25th day. The most obvious finding of the present study is that chitosan-collagen gel (3:1) represented a better efficacy compared to sulfadiazine in burn wound healing on day 25 post-burn.
本研究聚焦于烧伤，烧伤是全球导致死亡的主要原因之一，会产生有害的经济和社会影响。本研究的目的是调查从[具体物质]和虾壳中提取的胶原蛋白-壳聚糖凝胶对大鼠二度烧伤愈合的治疗效果。
为实现研究目的，分别采用酸法从虾和[具体物质]皮废料中提取壳聚糖和胶原蛋白，并使用Pico Tag、SDS-PAGE进行评估。利用大鼠在体内检测海洋胶原蛋白-壳聚糖凝胶的烧伤创面愈合效果。制备三种不同比例的胶原蛋白与壳聚糖混合物（Col-CH，1:3、1:1和3:1），以获得对烧伤创面愈合最有效的Col-CH凝胶，并与用磺胺嘧啶银软膏治疗的动物进行比较。通过用Image J测量创面面积来研究烧伤创面愈合情况，并在烧伤后第15天和第25天基于上皮化、成纤维细胞、急性和慢性炎症细胞、血管生成、胶原蛋白结构以及胶原蛋白含量的平均值进行组织病理学检查。
SDS-PAGE结果表明，提取的胶原蛋白为I型，由两条α链（α1和α2）组成。氨基酸分析显示，提取的胶原蛋白中甘氨酸含量高得多，占总氨基酸的三分之一。创面面积测量显示，与磺胺嘧啶治疗组相比，在第15天和第25天，Col-CH（3:1）治疗组的创面大小显著减小。组织病理学结果显示，在第25天，Col-CH（3:1）治疗组在上皮化、胶原蛋白、胶原蛋白结构、成纤维细胞方面得分较高，炎症细胞浸润减少。本研究最明显的发现是，在烧伤后第25天，壳聚糖-胶原蛋白凝胶（3:1）在烧伤创面愈合方面比磺胺嘧啶表现出更好的疗效。
