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茶皂素的纯化及其对乙醇脱氢酶活性影响的评价

Purification of Tea Saponins and Evaluation of its Effect on Alcohol Dehydrogenase Activity.

作者信息

Yuan Chuanxun, Li Yan, Li Qingchuan, Jin Risheng, Ren Lili

机构信息

Engineering Research Center of Bio-process (Hefei University of Technology), Ministry of Education, Hefei, Anhui 230009, P.R, China.

出版信息

Open Life Sci. 2018 Apr 10;13:56-63. doi: 10.1515/biol-2018-0008. eCollection 2018 Jan.

DOI:10.1515/biol-2018-0008
PMID:33817068
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7874680/
Abstract

Tea saponins, extracted from a cake, were found to have a potent effect on de-alcoholic activity. To obtain highly pure tea saponins, which can better maintain the activity of alcohol dehydrogenase (ADH), this paper presents an extraction method for tea saponins using deionized water as the extraction agent and a two-stage precipitation method, including ethanol precipitation and CaO precipitation. The optimum conditions for ethanol precipitation were 95% alcohol, a duration of 1.5h and a solid/liquid ratio of 1:4; while the optimum conditions for CaO precipitation were a duration of 2h and an NHHCO/CaO ratio of 2:1. Under the optimum conditions, the content of saponins was 87.58%. The results showed that the greater the amount of tea saponins and the higher its purity, the more significant its activating effect on ADH. When the purity of tea saponins was above 75%, it activated ADH. It indicated that the de-alcoholic mechanism of tea saponins is associated with the activity of ADH. Furthermore, the study characterized the structure of tea saponins by UV absorption and Fourier Transform Infrared (FTIR) spectrometry and LC-MS.

摘要

从茶饼中提取的茶皂苷被发现对解酒活性有显著作用。为了获得能更好地保持乙醇脱氢酶（ADH）活性的高纯度茶皂苷，本文提出了一种以去离子水为提取剂、采用乙醇沉淀和氧化钙沉淀两步沉淀法的茶皂苷提取方法。乙醇沉淀的最佳条件为95%乙醇、持续时间1.5小时和固液比1:4；而氧化钙沉淀的最佳条件为持续时间2小时和碳酸氢铵与氧化钙的比例为2:1。在最佳条件下，皂苷含量为87.58%。结果表明，茶皂苷的量越大、纯度越高，其对ADH的激活作用越显著。当茶皂苷纯度高于75%时，它能激活ADH。这表明茶皂苷的解酒机制与ADH的活性有关。此外，该研究通过紫外吸收、傅里叶变换红外（FTIR）光谱和液相色谱 - 质谱联用对茶皂苷的结构进行了表征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/ce1c597740cd/biol-13-056-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/bd090f31a0ef/biol-13-056-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/f51cfd23f3a2/biol-13-056-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/1fbba9dc880b/biol-13-056-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/35e239fffb8c/biol-13-056-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/716a71d926a8/biol-13-056-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/5d2cdfc093bd/biol-13-056-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/bd85279660c9/biol-13-056-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/c3d1c5feaab6/biol-13-056-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/8e21dee3b334/biol-13-056-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/ce1c597740cd/biol-13-056-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/bd090f31a0ef/biol-13-056-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/f51cfd23f3a2/biol-13-056-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/1fbba9dc880b/biol-13-056-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/35e239fffb8c/biol-13-056-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/716a71d926a8/biol-13-056-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/5d2cdfc093bd/biol-13-056-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/bd85279660c9/biol-13-056-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/c3d1c5feaab6/biol-13-056-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/8e21dee3b334/biol-13-056-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce9e/7874680/ce1c597740cd/biol-13-056-g010.jpg

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