Tang Wei, Luo Caroline
College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China.
Department of Microbiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Open Life Sci. 2018 Nov 9;13:431-445. doi: 10.1515/biol-2018-0052. eCollection 2018 Jan.
The purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of 6 () in salt stress tolerance in cells of rice ( L.), cotton ( L.) and slash pine ( Engelm.). Cells of , , and overexpressing were generated using -mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, also increased expression of Ca-dependent protein kinase genes 9 and 25 by 5-7 fold under NaCl stress. Altogether, our results suggest that overexpression of enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca-dependent protein kinase in transgenic cell lines of different plant species.
本研究的目的是检测6（）的C2H2型锌指转录因子在水稻（L.）、棉花（L.）和湿地松（Engelm.）细胞耐盐胁迫中的功能。使用介导的遗传转化产生了过表达的、和细胞。对转基因细胞系的分子和功能分析表明，过表达通过降低脂质过氧化、增加脱落酸（ABA）和赤霉素（GA）含量以及增强抗氧化酶如抗坏血酸过氧化物酶（APOX）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）的活性来提高对盐胁迫的耐受性。在水稻细胞中，在NaCl胁迫下，还使钙依赖蛋白激酶基因9和25的表达增加了5至7倍。总之，我们的结果表明，过表达通过提高不同植物物种转基因细胞系中的抗氧化酶活性、激素含量和钙依赖蛋白激酶的表达来增强盐胁迫耐受性。
