选定小麦品种中叶锈病抗性基因的鉴定及多重PCR技术的开发

Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR.

作者信息

Tomkowiak Agnieszka, Skowrońska Roksana, Buda Alicja, Kurasiak-Popowska Danuta, Nawracała Jerzy, Kowalczewski Przemysław Łukasz, Pluta Mateusz, Radzikowska Dominika

机构信息

Department of Genetic and Plant Breeding, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, Poland.

Institute of Food Technology of Plant Origin, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, 31 Wojska Polskiego Str, 60-624 Poznań, Poland.

出版信息

Open Life Sci. 2019 Jul 22;14:327-334. doi: 10.1515/biol-2019-0036. eCollection 2019 Jan.

DOI:10.1515/biol-2019-0036

PMID:33817166

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7874803/

Abstract

Ten leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the and genes encoding resistance to leaf rust using molecular SSR markers ( and ) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the and and and gene pairs.

摘要

2017年和2018年，选用了来自植物育种与驯化研究所（IHAR）——国家研究所（波兰）和基因库部门（捷克共和国）的10个主要小麦品种，在德隆实验农场开展了一项田间试验。所分析的小麦基因型具有对叶锈病的多样化田间抗性。2017年和2018年，Jubilatka、Thatcher和Sparta在田间条件下是抗性最强的品种。这项工作的目的是使用分子SSR标记（和）鉴定编码对叶锈病抗性的和基因，并开发多重PCR条件以加速这些基因的鉴定。在这些品种中同时鉴定出了三个叶锈病抗性基因的标记。Jubilatka、Thatcher和Sparta品种可能是所分析的叶锈病抗性基因的良好来源。此外，还开发了多重PCR条件，用于同时鉴定和以及和基因对。