Bobrowska Roksana, Moskalik Jakub, Noweiska Aleksandra, Spychała Julia, Tomkowiak Agnieszka, Kwiatek Michał Tomasz
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, 11 Dojazd Str, 60-632, Poznań, Poland.
Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute, 05-870 Błonie , Radzików, Poland.
J Appl Genet. 2025 Sep 13. doi: 10.1007/s13353-025-01004-z.
Resistance breeding is a widely promoted strategy for minimizing yield losses in wheat caused by various fungal diseases, including leaf rust (Puccinia triticina; Lr genes), Fusarium head blight (Fusarium spp.; Fhb), powdery mildew (Blumeria graminis; Pm), Septoria tritici blotch (Septoria tritici; Stb), eyespot (Oculimacula spp.; Pch-previously known as Pseudocercosporella herpotrichoides), stem rust (Puccinia graminis f. sp. tritici; Sr) and yellow rust (Puccinia striiformis; Yr). Understanding the prevalence of resistance genes in currently cultivated European varieties is crucial for their effective utilization in breeding programs. In this study, we developed 11 duplex and 13 triplex PCR assays for the simultaneous identification of diverse allelic combinations of disease resistance genes. Selected assays were used to analyze 70 European wheat varieties for the presence of 15 molecular markers associated with 12 resistance genes. The analyses identified diverse resistance gene combinations. The optimized multiplex PCR methods significantly reduce cost and time of analysis, making them valuable tools for marker-assisted selection (MAS) in wheat breeding programs.
抗病育种是一种广泛推广的策略，用于尽量减少各种真菌病害导致的小麦产量损失，这些病害包括叶锈病（小麦条锈菌；Lr基因）、赤霉病（镰刀菌属；Fhb）、白粉病（禾本科布氏白粉菌；Pm）、小麦叶枯病（小麦壳针孢；Stb）、眼斑病（黄斑病菌属；Pch - 以前称为小麦假尾孢菌）、杆锈病（小麦柄锈菌；Sr）和条锈病（条形柄锈菌；Yr）。了解当前种植的欧洲品种中抗病基因的流行情况对于在育种计划中有效利用这些基因至关重要。在本研究中，我们开发了11种双重PCR和13种三重PCR检测方法，用于同时鉴定抗病基因的不同等位基因组合。选用的检测方法用于分析70个欧洲小麦品种，以确定与12个抗病基因相关的15个分子标记的存在情况。分析确定了不同的抗病基因组合。优化后的多重PCR方法显著降低了分析成本和时间，使其成为小麦育种计划中标记辅助选择（MAS）的宝贵工具。
