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冠状动脉粒细胞集落刺激因子对慢性缺血性心脏病大鼠的治疗作用。

Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease.

作者信息

Ren Pengcheng, Zhang Ming, Dai Shuren

机构信息

Department of Cardiology, Chongqing Dazu District People's Hospital, Chongqing, 402360, People's Republic of China.

出版信息

Open Life Sci. 2020 Oct 20;15(1):742-752. doi: 10.1515/biol-2020-0078. eCollection 2020.

DOI:10.1515/biol-2020-0078
PMID:33817262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7747518/
Abstract

BACKGROUND

The aim of this study was to evaluate the therapeutic effects of coronary granulocyte colony-stimulating factor (G-CSF) on rats with chronic ischemic heart disease (CIHD).

METHODS

Thirty healthy rats were randomly divided into control, subcutaneous and intracoronary G-CSF injection groups ( = 10) after the CIHD model was established. Left ventricular ejection fraction (LVEF), myocardial injury area, myocardial perfusion area and viable myocardium were observed by coronary angiography, dual-isotopic myocardial imaging and first-pass delayed myocardial perfusion magnetic resonance imaging (MRI) before modeling as well as 2 and 4 weeks after surgery.

RESULTS

The peak times of peripheral blood and subcutaneous G-CSF levels were 3 and 5 days after mobilization, respectively. The peripheral blood CD34+/CD133+ cell ratio of subcutaneous or intracoronary G-CSF injection group significantly exceeded that of the control group ( < 0.05). The distal stenosis degrees of target lesions in subcutaneous and intracoronary G-CSF injection groups were significantly lower than that of the control group ( < 0.05). Compared with the situation before mobilization, LVEF was significantly improved after 2 weeks in intracoronary and subcutaneous G-CSF injection groups ( < 0.01). Their infarcted myocardial areas were reduced, the left ventricular remodeling was relieved, the percentage of viable myocardium was increased, angiogenesis was promoted and cardiomyocyte apoptosis was inhibited.

CONCLUSION

Intracoronary G-CSF injection is safe and effective as subcutaneous injection, improving the cardiac function of CIHD rats.

摘要

背景

本研究旨在评估冠状动脉注射粒细胞集落刺激因子（G-CSF）对慢性缺血性心脏病（CIHD）大鼠的治疗效果。

方法

30只健康大鼠在建立CIHD模型后随机分为对照组、皮下注射G-CSF组和冠状动脉内注射G-CSF组（每组 = 10只）。在建模前以及术后2周和4周，通过冠状动脉造影、双同位素心肌显像和首过延迟心肌灌注磁共振成像（MRI）观察左心室射血分数（LVEF）、心肌损伤面积、心肌灌注面积和存活心肌。

结果

外周血和皮下G-CSF水平的峰值时间分别为动员后3天和5天。皮下或冠状动脉内注射G-CSF组的外周血CD34+/CD133+细胞比例显著高于对照组（ < 0.05）。皮下和冠状动脉内注射G-CSF组靶病变的远端狭窄程度显著低于对照组（ < 0.05）。与动员前相比，冠状动脉内和皮下注射G-CSF组在2周后LVEF显著改善（ < 0.01）。它们的梗死心肌面积减小，左心室重构减轻，存活心肌百分比增加，血管生成增加，心肌细胞凋亡受到抑制。

结论

冠状动脉内注射G-CSF与皮下注射一样安全有效，可改善CIHD大鼠的心功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/ef2a95bf489f/j_biol-2020-0078-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/591e56a999d4/j_biol-2020-0078-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/dba381160fdf/j_biol-2020-0078-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/e4270c4bc3ab/j_biol-2020-0078-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/ceabfa041d4d/j_biol-2020-0078-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/677ee2394de3/j_biol-2020-0078-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/5ea938e1b7ec/j_biol-2020-0078-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/ef2a95bf489f/j_biol-2020-0078-fig007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/591e56a999d4/j_biol-2020-0078-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/dba381160fdf/j_biol-2020-0078-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/e4270c4bc3ab/j_biol-2020-0078-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/ceabfa041d4d/j_biol-2020-0078-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/677ee2394de3/j_biol-2020-0078-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/5ea938e1b7ec/j_biol-2020-0078-fig006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/917e/7747518/ef2a95bf489f/j_biol-2020-0078-fig007.jpg

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