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晚期胚胎丰富(LEA)基因家族在:鉴定、表达分析和对干旱胁迫的响应。

Late embryogenesis abundant (LEA) gene family in : identification, expression analysis, and response to drought stress.

机构信息

Shaanxi Academy of Traditional Chinese Medicine, Xi'an Shaanxi, China.

College of Agriculture, Henan University of Science and Technology, Luoyang Henan, China.

出版信息

Plant Signal Behav. 2021 May 4;16(5):1891769. doi: 10.1080/15592324.2021.1891769. Epub 2021 Apr 5.

DOI:10.1080/15592324.2021.1891769
PMID:33818288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8078505/
Abstract

Late embryogenesis abundant (LEA) proteins play important roles in plant defense response to drought stress. However, genome-wide identification of the gene family was not revealed in . In this study, 61 genes were identified from and divided into 7 subfamilies according to their conserved domains and phylogenetic relationships. genes contained the LEA conserved motifs and few introns. genes of the same subfamilies had similar gene structures and predicted subcellular locations. Our results indicated that the promoters of genes contained various -acting elements associated with abiotic stress response. In addition, RNA-seq and real-time PCR results suggested that genes are specifically expressed in different tissue, and most genes can be induced by drought stress. These results provide a valuable foundation for future functional investigations of genes and drought stress-resistant breeding of .

摘要

晚期胚胎丰富(LEA)蛋白在植物对干旱胁迫的防御反应中起着重要作用。然而,在 中尚未揭示基因家族的全基因组鉴定。在这项研究中,从 中鉴定出 61 个基因,并根据其保守结构域和系统发育关系分为 7 个亚家族。 基因含有 LEA 保守基序和少数内含子。同一亚家族的 基因具有相似的基因结构和预测的亚细胞定位。我们的结果表明, 基因的启动子含有与非生物胁迫反应相关的各种 - 作用元件。此外,RNA-seq 和实时 PCR 结果表明, 基因在不同组织中特异性表达,大多数 基因可以被干旱胁迫诱导。这些结果为进一步研究 基因的功能和 的抗旱性育种提供了有价值的基础。

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