Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6 St., 44-100 Gliwice, Poland.
Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6 St., 44-100 Gliwice, Poland.
Food Chem. 2021 Sep 15;356:129680. doi: 10.1016/j.foodchem.2021.129680. Epub 2021 Mar 25.
Liquid chromatography coupled with tandem mass spectrometry was employed for the detection of pesticides (thiamethoxam, lambda-cyhalothrin, deltamethrin, and metalaxyl) and their metabolites in Raphanus sativus var. longipinnatus exposed to these compounds under experimental conditions. Metalaxyl (0.008 mg/kg), metalaxyl acid (0.009 mg/kg), and (+)-trans-chrysanthemic acid (0.098 mg/kg) were identified in the plants exposed to the individual pesticides and their metabolites. Non-targeted analysis revealed the presence of thiamethoxam, lambda-cyhalothrin, and deltamethrin metabolites in plants exposed to these substances, despite the fact that the pesticide concentrations were below the analytical method's limit of quantification (0.005-0.006 mg/kg). Based on the non-targeted screening, non-specific (leucine and tyramine) and specific (epinephrine, dopamine, tryptamine, and serotonin) markers of plant exposure to the mentioned stress-inducing compounds were detected. These findings prove that non-targeted analysis is an indispensable tool for determining plants' exposure to pesticides, even when the parent compound has been completely metabolized.
采用液相色谱-串联质谱法检测了萝卜在实验条件下暴露于这些化合物时的农药（噻虫嗪、高效氯氟氰菊酯、溴氰菊酯和甲霜灵）及其代谢物。在暴露于单一农药及其代谢物的植物中，检测到了金属axyl（0.008mg/kg）、金属axyl 酸（0.009mg/kg）和（+）-反式菊酸（0.098mg/kg）。非靶向分析显示，尽管农药浓度低于分析方法的定量限（0.005-0.006mg/kg），但在暴露于这些物质的植物中仍存在噻虫嗪、高效氯氟氰菊酯和溴氰菊酯的代谢物。基于非靶向筛选，检测到了植物暴露于所述应激诱导化合物的非特异性（亮氨酸和酪胺）和特异性（肾上腺素、多巴胺、色胺和血清素）标志物。这些发现证明，即使母体化合物已完全代谢，非靶向分析仍是确定植物暴露于农药的不可或缺的工具。
