Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6, 44-100 Gliwice, Poland.
Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6, 44-100 Gliwice, Poland; School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, PR China.
Food Chem. 2021 Jan 15;335:127658. doi: 10.1016/j.foodchem.2020.127658. Epub 2020 Jul 25.
Raphanus sativus var. longipinnatus, was exposed under experimental conditions to herbicides: rimsulfuron (RIM), administrated as (1) pure substance, (2) in commercially available formulation (RIMEL), (3) its degradation product: 4,6-dimethoxypyrimidin-2-amine (2ADP), (4) mesotrione (MES), (5) sulcotrione (SUL). Profiling and fingerprinting strategies, conducted by LC-MS/MS-FL, were employed to find markers of plant exposure to herbicide stress. The presence ofRIM metabolite in the tissues of plant exposed to this herbicide proved that it is necessary to determine both parent compound and its by-products to obtain reliable information on plant exposure to agrochemicals. A higher content of normetanephrine (NMN) (18-175%) and lower content of tyramine (TYR) (49-75%) and epinephrine (E) (75-83%) was observed in plant tissues exposed to RIM and 2ADP in comparison to blank sample. Therefore, NMN, TRY and E may be considered as markers of plant response to RIM. Non-target analysis enables to recognize the type of herbicide used during cultivation.
啶嘧磺隆（RIM），以（1）纯物质，（2）市售制剂（RIMEL），（3）其降解产物：4,6-二甲氧基嘧啶-2-胺（2ADP），（4）唑草酮（MES），（5）砜嘧磺隆（SUL）进行处理。采用 LC-MS/MS-FL 进行的剖析和指纹图谱策略，用于寻找植物暴露于除草剂胁迫的标志物。暴露于该除草剂的植物组织中 RIM 代谢物的存在证明，有必要同时测定母体化合物及其副产物，以获得有关植物接触农药的可靠信息。与空白样品相比，暴露于 RIM 和 2ADP 的植物组织中检测到较高含量的去甲肾上腺素（NMN）（18-175％）和较低含量的酪胺（TYR）（49-75％）和肾上腺素（E）（75-83％）。因此，NMN、TRY 和 E 可以被认为是植物对 RIM 反应的标志物。非靶向分析能够识别在种植过程中使用的除草剂类型。
