Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia.
Institute of Pesticides and Environmental Protection, Beograd-Zemun, Serbia.
Environ Technol. 2022 Aug;43(19):3000-3013. doi: 10.1080/09593330.2021.1913241. Epub 2021 Apr 18.
Search for more environment-friendly methods for controlling plant diseases that would contribute to the goal of sustainability in agriculture is in focus. In the present study, the potential of isolated from soil sample in the production of biocontrol agents, nigericin and niphimycin, effective against storage apple pathogen was examined. Also, modelling and optimization of medium composition for biocontrol agent biosynthesis was performed. The results showed that the optimum amount of CHO, (NH)SO and KHPO in the medium for biosynthesis is 20, 0.25 and 1.46 g/L, respectively. and validation of the obtained results performed in the 3 L laboratory-scale bioreactor showed that on the optimized medium at an aeration rate of 0.7 vvm and an agitation speed of 200 rpm, produced nigericin and niphimycin, showed high activity. Under the same conditions, cultivation of was performed in a 7 L laboratory bioreactor in a medium with waste glycerol instead of pure glycerol. Results showed that the methanol extract of cultivation liquid, containing nigericin and niphimycin, was high effective against two isolates. This was confirmed by obtaining large inhibition zone diameters on KA10 (47 mm) and T1Jg (44.33 mm) isolates. After successful analysis, testing was performed. It was found that necrosis diameters that were measured on artificially inoculated apple fruits with compared to necrosis diameter measured on untreated, control fruits, were 4.47 and 3.56 times smaller.
寻找更环保的方法来控制植物病害，以促进农业的可持续性目标，这是当前的研究重点。在本研究中，从土壤样本中分离出的，对储存苹果病原体有效的生物防治剂 Nigericin 和 Niphimycin 进行了生产潜力的研究。此外，还对生物防治剂生物合成的培养基组成进行了建模和优化。结果表明，CHO、(NH4)2SO4 和 KH2PO4 在培养基中最适的生物合成量分别为 20、0.25 和 1.46 g/L。在 3 L 实验室规模生物反应器中对获得的结果进行了验证，结果表明，在优化的培养基中，通气率为 0.7 vvm，搅拌速度为 200 rpm 时，产生的 Nigericin 和 Niphimycin 表现出很高的活性。在相同条件下，在含有废甘油的培养基中，在 7 L 实验室生物反应器中进行了 的培养。结果表明，含有 Nigericin 和 Niphimycin 的 培养液的甲醇提取物对两种 分离株具有很高的抑制活性。这一点通过在 KA10（47 mm）和 T1Jg（44.33 mm）分离株上获得大的抑菌圈直径得到了证实。成功进行了 分析后，进行了 测试。结果发现，与未处理的对照果实相比，用 处理的人工接种苹果果实上测量到的坏死直径分别小 4.47 和 3.56 倍。
