New D A, Cockroft D L
Experientia. 1979 Jan 15;35(1):138-40. doi: 10.1007/BF01917926.
A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.
本文描述了一种“旋转器”培养方法,该方法能为旋转瓶中的培养物提供持续的含氧气体流。在整个培养期间,该系统可维持培养基中氧气和二氧化碳水平恒定。与密封培养瓶系统相比,它还能提供更稳定的pH值。