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一种气相连续置换的旋转瓶培养方法。

A rotating bottle culture method with continuous replacement of the gas phase.

作者信息

New D A, Cockroft D L

出版信息

Experientia. 1979 Jan 15;35(1):138-40. doi: 10.1007/BF01917926.

DOI:10.1007/BF01917926
PMID:33822
Abstract

A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.

摘要

本文描述了一种“旋转器”培养方法,该方法能为旋转瓶中的培养物提供持续的含氧气体流。在整个培养期间,该系统可维持培养基中氧气和二氧化碳水平恒定。与密封培养瓶系统相比,它还能提供更稳定的pH值。

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A rotating bottle culture method with continuous replacement of the gas phase.一种气相连续置换的旋转瓶培养方法。
Experientia. 1979 Jan 15;35(1):138-40. doi: 10.1007/BF01917926.
2
The culture of 12- and 13-day rat embryos using continuous and noncontinuous gassing of rotating bottles.
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本文引用的文献

1
Culture of explanted rat embryos in rotating tubes.将取出的大鼠胚胎置于旋转管中培养。
J Reprod Fertil. 1973 Oct;35(1):135-8. doi: 10.1530/jrf.0.0350135.
2
Direct exposure of postimplantation mouse embryos to 5-bromodeoxyuridine in vitro and its effect on subsequent chondrogenesis in the limbs.植入后小鼠胚胎在体外直接暴露于5-溴脱氧尿苷及其对四肢后续软骨形成的影响。
J Embryol Exp Morphol. 1976 Dec;36(3):623-38.
3
Comparison of growth in vitro and in vivo of post-implantation rat embryos.
鼠胚整体培养:评估鼠血清作为培养基的需求。
Birth Defects Res. 2019 Oct 1;111(16):1165-1177. doi: 10.1002/bdr2.1538. Epub 2019 Jun 24.
4
Thalidomide-induced limb abnormalities in a humanized CYP3A mouse model.沙利度胺在人源化CYP3A小鼠模型中诱导的肢体异常。
Sci Rep. 2016 Feb 23;6:21419. doi: 10.1038/srep21419.
5
Interferon regulatory factor 6 regulates keratinocyte migration.干扰素调节因子6调控角质形成细胞迁移。
J Cell Sci. 2014 Jul 1;127(Pt 13):2840-8. doi: 10.1242/jcs.139246. Epub 2014 Apr 28.
6
Mouse embryonic development in a serum-free whole embryo culture system.在无血清全胚胎培养系统中的小鼠胚胎发育
J Vis Exp. 2014 Mar 1(85):50803. doi: 10.3791/50803.
7
The method of rodent whole embryo culture using the rotator-type bottle culture system.使用旋转式瓶培养系统进行啮齿动物全胚胎培养的方法。
J Vis Exp. 2010 Aug 28(42):2170. doi: 10.3791/2170.
8
Imaging mouse embryonic development.小鼠胚胎发育成像。
Methods Enzymol. 2010;476:329-49. doi: 10.1016/S0076-6879(10)76019-5.
9
A morphological study of the development of the allantois of rat embryos in vivo.大鼠胚胎尿囊在体内发育的形态学研究。
J Anat. 1985 Oct;142:1-11.
10
Effects of fibroblast growth factor 2 and insulin-like growth factor II on the development of parthenogenetic mouse embryos in vitro.成纤维细胞生长因子2和胰岛素样生长因子II对孤雌生殖小鼠胚胎体外发育的影响。
In Vitro Cell Dev Biol Anim. 2001 Jul-Aug;37(7):440-4. doi: 10.1290/1071-2690(2001)037<0440:EOFGFA>2.0.CO;2.
J Embryol Exp Morphol. 1976 Aug;36(1):133-44.
4
Regeneration of amputated limb-buds in early rat embryos.
J Embryol Exp Morphol. 1976 Apr;35(2):345-54.
5
In vitro development of early postimplantation rat embryos.植入后早期大鼠胚胎的体外发育
Dev Biol. 1978 Aug;65(2):396-403. doi: 10.1016/0012-1606(78)90035-0.
6
The use of in vitro procedures in teratology.体外程序在致畸学中的应用。
Teratology. 1975 Jun;11(3):273-87. doi: 10.1002/tera.1420110307.